D0550
3,5-二硝基水杨酸
used in colorimetric determination of reducing sugars
别名:
2-羟基-3,5-二硝基苯甲酸, DNS
质量水平
方案
≥98% (HPLC and titration)
表单
powder
mp
168-172 °C (lit.)
溶解性
H2O: 50 mg/mL
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
InChI key
LWFUFLREGJMOIZ-UHFFFAOYSA-N
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应用
已经使用 3，5-二硝基水杨酸（DNS）来停止体外 α-淀粉酶抑制研究中的反应。它还用于酶促释放的还原糖的定量。
生化/生理作用
3，5-二硝基水杨酸（DNS）用于还原糖的比色测定，并通过定量酶释放的还原糖来分析糖苷酶（糖苷水解酶）活性。已经将二硝基水杨酸方法与 Nelson-Somogi 比色法进行了比较。
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Oral - Eye Dam. 1
储存分类代码
4.1A - Other explosive hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.
Mokni-Tlili S, et al.
World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)
Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.
J F Robyt et al.
Analytical biochemistry, 45(2), 510-516 (1972-02-01)
Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.
Breuil, C. and Saddler, J.N.
Enzyme and Microbial Technology, 7, 327-332 (1985)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)
Rammohan Subramanian et al.
Acta biochimica Polonica, 55(2), 391-398 (2008-05-31)
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro
实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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