D4418

3,3′-Diaminobenzidine

Name: 3,3′-Diaminobenzidine
Brand: SIGMA

SIGMAFAST^™, chromogenic, tablet

别名:

DAB, DAB tablets, Immunohistology substrate

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关于此项目

化学文摘社编号:

91-95-2

MDL编号:

MFCD00007725

UNSPSC代码:

12352204

PubChem化学物质编号:

24893872

NACRES:

NA.83

主要文件

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产品名称

SIGMAFAST^™ 3,3′-二氨基联苯胺片, tablet, To prepare 15 mL

表单

tablet

溶解性

water: 1 tablet/5 mL

储存温度

−20°C

SMILES字符串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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应用

本品适于作为沉淀底物，在免疫组织和免疫印迹分析中原位检测过氧化物酶活性。DAB会生成高强度的耐酒精黑棕色色斑，是免疫组织分析的首选底物。经DAB染色后，玻片可进一步进行脱水和树脂封片处理，以便保存供后续比对。SIGMAFAST^™ DAB片剂不需要另外的缓冲液或步骤来配制活性底物溶液。铝箔独立包装。

制备说明

将每片片剂溶于 15 mL 去离子水中，得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

象形图

GHS08,GHS05,GHS07

警示用语：

Danger

危险声明

H302,H315,H318,H335,H341,H350

预防措施声明

P202 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313

危险分类

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Truncation of mutant huntingtin in knock-in mice demonstrates exon1 huntingtin is a key pathogenic form.

Huiming Yang et al.

Nature communications, 11(1), 2582-2582 (2020-05-24)

Polyglutamine expansion in proteins can cause selective neurodegeneration, although the mechanisms are not fully understood. In Huntington's disease (HD), proteolytic processing generates toxic N-terminal huntingtin (HTT) fragments that preferentially kill striatal neurons. Here, using CRISPR/Cas9 to truncate full-length mutant HTT

ZENK expression following conspecific and heterospecific playback in the zebra finch auditory forebrain.

Erin N Scully et al.

Behavioural brain research, 331, 151-158 (2017-05-17)

Zebra finches (Taeniopygia guttata) are sexually dimorphic songbirds, not only in appearance but also in vocal production: while males produce both calls and songs, females only produce calls. This dimorphism provides a means to contrast the auditory perception of vocalizations

Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants.

Laura Riva et al.

Pathogens (Basel, Switzerland), 10(2) (2021-02-10)

The hepatitis C virus (HCV) life cycle is a tightly regulated process, during which structural and non-structural proteins cooperate. However, the interplay between HCV proteins during genomic RNA replication and progeny virion assembly is not completely understood. Here, we studied

Post-acute delivery of memantine promotes post-ischemic neurological recovery, peri-infarct tissue remodeling, and contralesional brain plasticity.

Ya-Chao Wang et al.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 980-993 (2016-05-14)

The NMDA antagonist memantine preferentially inhibits extrasynaptic NMDA receptors, which are overactivated upon stroke and thought to disturb neuroplasticity. We hypothesized that memantine enhances post-ischemic neurological recovery, brain remodeling, and plasticity. C57BL6/j mice were exposed to intraluminal middle cerebral artery

Phosphorylation of STAT1 serine 727 enhances platinum resistance in uterine serous carcinoma.

Xiang Zeng et al.

International journal of cancer, 145(6), 1635-1647 (2019-06-23)

Uterine serous carcinoma (USC) is a highly aggressive histological subtype of endometrial cancers harboring highly metastatic and chemoresistant features. Our previous study showed that STAT1 is highly expressed in USC and acts as a key molecule that is positively correlated

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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

3,3′-Diaminobenzidine

SIGMAFAST^™, chromogenic, tablet