选择尺寸
关于此项目
biological source
fish testis (salmon)
Quality Level
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
concentration
10 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
General description
Application
为了减少探针与基质的任何非特异性杂交,必须使用封闭剂。 通常,使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA,可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
Preparation Note
Other Notes
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
此项目有
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
商品
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
转化是将外源DNA引入细胞,引起遗传变异或基因修饰的过程。1928年,Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
相关内容
Instructions
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D9156-1ML | 04061833590355 |
| D9156-5ML | 04061833590362 |
| D9156-5X1ML | 04061833590379 |