Merck
CN

D9156

Sigma-Aldrich

脱氧核糖核酸,单链 来源于鲑鱼睾丸

For hybridization

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别名:
single-stranded template DNA
CAS号:
MDL编号:
eCl@ss:
32160414
NACRES:
NA.52

生物来源

fish testis (salmon)

质量水平

等级

for molecular biology

检测方案

9-11 mg/mL (DNA concentration)

形式

solution

浓度

10 mg/mL in H2O

运输

dry ice

储存温度

−20°C

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此商品
D7656D8899D4416
grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

assay

9-11 mg/mL (DNA concentration)

assay

9-11 mg/mL

assay

-

assay

-

form

solution

form

solution

form

lyophilized powder

form

solution

concentration

10 mg/mL in H2O

concentration

10.0 ± 1.0 mg/mL in H2O

concentration

-

concentration

-

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

wet ice

一般描述

是从鲑鱼睾丸分离优质单链模板DNA的即用型解决方案。 溶液的供应浓度为9-12 mg/ml。

应用

许多因素有助于提高核酸杂交的信噪比。 这些因素包括溶剂(甲酰胺)的存在、杂交温度、杂交长度、杂交溶液体积、搅拌程度和方法、封闭剂的使用、探针浓度和比活性、使用分子试剂提高核酸重缔合的速率以及在洗涤膜的严格程度。

为了减少探针与基质的任何非特异性杂交,必须使用封闭剂。 通常,使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA,可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
鲑鱼睾丸的单链脱氧核糖核酸适合用作Southern杂交中的封闭剂。用于骨髓活检样本 和人血单核细胞亚群的原位杂交组织化学研究。

制备说明

将该DNA用乙醇沉淀并超声处理,产生单链片段,其与587和831碱基对标记片段共迁移。

其他说明

DNA溶液要在室温下放置进行再退火,因此建议使用前将溶液煮沸10分钟,然后在冰上至少冷却5分钟。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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