General description
凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷，并且与DNA的迁移方向相同，从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1：6稀释。
Application
适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳（PAGE），该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。
Preparation Note
凝胶上样缓冲液含有0.25％溴酚蓝、0.25％二甲苯蓝和40％蔗糖。
Other Notes
预期条带迁移如下：
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
Datasheet
David R Macinga et al.
Antimicrobial agents and chemotherapy, 47(8), 2526-2537 (2003-07-25)
We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
S Henry et al.
Vox sanguinis, 70(1), 21-25 (1996-01-01)
While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G7654-5ML | 04061838099358 |