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安全信息

H9773

Sigma-Aldrich

潮霉素B 来源于吸水链霉菌

suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder

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别名:
Hygromycin
经验公式（希尔记法）:
C20H37N3O13
CAS号:
31282-04-9
分子量:
527.52
EC 号:
250-545-5
MDL编号:
MFCD06795479
PubChem化学物质编号:
329815270
NACRES:
NA.72

生物来源

Streptomyces hygroscopicus

质量水平

200

产品线

BioReagent

形式

lyophilized powder

纯化方式

ion-exchange chromatography

浓度

≥60% (HPLC)

技术

cell culture | plant: suitable

颜色

faintly brown to brown
 white to beige

 

(1) 7.1, (2) 8.8

溶解性

H2O: soluble 50 mg/mL
ethanol: soluble
methanol: soluble

抗生素抗菌谱

fungi

应用

agriculture

作用机制

protein synthesis | interferes

储存温度

2-8°C

SMILES字符串

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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相关类别

一般描述

Chemical structure: aminoglycoside
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

应用

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.

生化/生理作用

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.
作用机制：本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。

抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作用。

注意

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

制备说明

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

象形图

Skull and crossbones
GHS06

警示用语：

Danger

危险声明

H300 + H310 + H330

危险分类

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Yajun Xi et al.
Methods in molecular biology (Clifton, N.J.), 581, 53-59 (2009-09-22)
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced
M Dutt et al.
Plant cell reports, 29(11), 1251-1260 (2010-08-17)
A method for the genetic transformation of several citrus cultivars is described, including cultivars observed to be recalcitrant to conventional epicotyl-mediated transformation. Embryogenic cell suspension cultures, established from unfertilized ovules were used as target tissues for Agrobacterium-mediated transformation. Several modifications
Inja Radman et al.
PloS one, 8(10), e76019-e76019 (2013-10-17)
We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with
Tsuyoshi Nakagawa et al.
Journal of bioscience and bioengineering, 104(1), 34-41 (2007-08-19)
We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and
Daisuke Iino et al.
Journal of structural biology, 183(1), 76-85 (2013-06-12)
Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) inactivates the aminoglycoside antibiotic hygromycin B (hygB) via phosphorylation. The crystal structure of the binary complex of APH(4)-Ia with hygB was recently reported. To characterize substrate recognition by the enzyme, we determined the crystal
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Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere with the processes at the 30S subunit or 50S subunit of the 70S bacterial ribosome.

抗生素对蛋白质合成的抑制作用

蛋白质合成是一个复杂、多步骤过程，涉及到很多酶以及构象排列。但是，阻碍细菌蛋白质合成的大部分抗生素对该过程的干扰发生在70S细菌核糖体的30S亚基或50S亚基。

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Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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