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H9773

潮霉素B 来源于吸水链霉菌

Name: 潮霉素B 来源于<I>吸水链霉菌</I>
Brand: SIGMA

lyophilized powder, suitable for plant cell culture, BioReagent

别名:

Hygromycin

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About This Item

经验公式（希尔记法）:

C₂₀H₃₇N₃O₁₃

CAS Number:

31282-04-9

分子量:

527.52

EC 号:

250-545-5

MDL编号:

MFCD06795479

UNSPSC代码:

12352207

PubChem化学物质编号:

329815270

NACRES:

NA.72

Product Name

潮霉素B 来源于吸水链霉菌, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder

生物来源

Streptomyces hygroscopicus

质量水平

200

产品线

BioReagent

表单

lyophilized powder

纯化方式

ion-exchange chromatography

浓度

≥60% (HPLC)

技术

cell culture | plant: suitable

颜色

faintly brown to brown
white to beige

(1) 7.1, (2) 8.8

溶解性

H₂O: soluble 50 mg/mL
ethanol: soluble
methanol: soluble

抗生素抗菌谱

fungi

应用

agriculture

作用机制

protein synthesis | interferes

储存温度

2-8°C

SMILES字符串

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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一般描述

Chemical structure: aminoglycoside

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

应用

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.

生化/生理作用

作用机制：本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。

抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作用。

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.

注意

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

制备说明

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.