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安全信息

L0418

Sigma-Aldrich

LB Agar Carbenicillin - 100, Plates

pre-poured agar plates with 100 μg/ml carbenicillin

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别名:
carbenicillin agar plates

等级

for molecular biology

质量水平

100

无菌性

sterile; autoclaved

形式

plates (10 cm)
plates (10cm, 20-25ml each)

存货情况

available only in USA

容量

20-25 mL

储存温度

2-8°C

适用性

selective for Escherichia coli
selective for coliforms

一般描述

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

应用

LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

特点和优势

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

组分

15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin

其他说明

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

相关产品

产品编号
说明
价格

C3416

羧苄青霉素 二钠盐, BioReagent, suitable for plant cell culture

L3147

含琼脂的 LB 肉汤 (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

L5667

LB Agar Ampicillin-100, Plates, pre-poured LB agar plates with 100 μg/ml ampicillin

L0168

LB Agar Ampicillin - 50, Plates, pre-poured LB agar plates with 50 μg/ml ampicillin

CMC0001

SIG10 化学感受态细胞, for protein expression and DNA plasmid production

CMC0007

SIG10 5a化学感受态细胞, for DNA plasmid production

CMC0014

BL21（DE3）化学感受态细胞, for protein expression

CMC0017

过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

储存分类代码

13 - Non Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why do the LB-agar plates with antibiotics have such a short expiration date?

    The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)

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