R6750
核糖核酸 来源于面包酵母（酿酒酵母）
别名:
核糖核酸
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关于此项目
Form:
powder
Solubility:
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
Storage temp.:
−20°C
Quality Level
form
powder
solubility
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
suitability
suitable for (suitable for use as a substrate for Rnase)
storage temp.
−20°C
General description
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）是RNase酶的底物。 从酵母中分离RNA是一个复杂的过程，涉及在苯和洗涤剂（SDS）环境中对细胞进行加热和冷冻循环。
Application
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）适用于Northern印迹杂交、逆转录聚合酶链式反应（RTPCR）和cDNA构建等应用。
Preparation Note
通过 Crestfield, A.M.等人的修改版本制备，J. Biol. Chem., 216, 185 (1955)。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R6750-100MG | 04061836695934 |
| R6750-500MG | 04061833290033 |
| R6750-1G | 04061836695941 |
| R6750-250MG | 04061833290026 |