R6750
核糖核酸 来源于面包酵母(酿酒酵母)
别名:
核糖核酸
一般描述
来自面包酵母(S. cerevisiae)的核糖核酸(RNA)是RNase酶的底物。 从酵母中分离RNA是一个复杂的过程,涉及在苯和洗涤剂(SDS)环境中对细胞进行加热和冷冻循环。
应用
来自面包酵母(S. cerevisiae)的核糖核酸(RNA)适用于Northern印迹杂交、逆转录聚合酶链式反应(RTPCR)和cDNA构建等应用。
制备说明
通过 Crestfield, A.M.等人的修改版本制备,J. Biol. Chem., 216, 185 (1955)。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Hidekazu Iioka et al.
Nucleic acids research, 39(8), e53-e53 (2011-02-09)
The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins.
Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Lukas Bossaller et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1044-1053 (2016-06-30)
Systemic lupus erythematosus (SLE) is a chronic, life-threatening autoimmune disorder, leading to multiple organ pathologies and kidney destruction. Analyses of numerous murine models of spontaneous SLE have revealed a critical role for endosomal TLRs in the production of autoantibodies and
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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