R6750
核糖核酸 来源于面包酵母（酿酒酵母）
别名:
核糖核酸
一般描述
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）是RNase酶的底物。 从酵母中分离RNA是一个复杂的过程，涉及在苯和洗涤剂（SDS）环境中对细胞进行加热和冷冻循环。
应用
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）适用于Northern印迹杂交、逆转录聚合酶链式反应（RTPCR）和cDNA构建等应用。
制备说明
通过 Crestfield, A.M.等人的修改版本制备，J. Biol. Chem., 216, 185 (1955)。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Douglas A Weigent
Cellular immunology, 282(2), 71-78 (2013-06-19)
In the present study, we show that higher levels of lymphocyte GH are expressed in spleen cells from aging animals compared to young animals. Further, leukocytes from primary and secondary immune tissues and splenic T and B cells from aging
Yajuan Qi et al.
Circulation. Heart failure, 8(1), 198-208 (2014-12-06)
Heart failure is a leading cause of morbidity and mortality in the USA and is closely associated with diabetes mellitus. The molecular link between diabetes mellitus and heart failure is incompletely understood. We recently demonstrated that insulin receptor substrates 1
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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