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T1503

Sigma-Aldrich

Trizma ® 碱

Primary Standard and Buffer, ≥99.9% (titration), crystalline

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别名:
2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇
线性分子式:
NH2C(CH2OH)3
CAS号:
77-86-1
分子量:
121.14
Beilstein:
741883
EC 号:
201-064-4
MDL编号:
MFCD00004679
PubChem化学物质编号:
24899973
NACRES:
NA.25

质量水平

300

描述

aminopeptidase substrate

检测方案

≥99.9% (titration)

形式

crystalline

储存条件

dry at room temperature

技术

ELISA: suitable
protein extraction: suitable

颜色

white

pH值（酸碱度）

10.5-12

有效pH范围

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

溶解性

methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)

吸光度

≤0.05 at 290 nm at 40%

适用性

suitable for Western blot
suitable for electrophoresis

应用

cell analysis
diagnostic assay manufacturing
life science and biopharma

SMILES字符串

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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相关类别

一般描述

三（羟甲基）氨基甲烷通常称为 Trometamol、Tris 碱或 Trizma® 碱，作为生物缓冲剂，在各种研究应用中发挥着关键作用。其最佳 pKa 为 8.1，使其成为配制 Tris-乙酸盐-EDTA (TAE) 和 Tris-硼酸盐-EDTA (TBE) 等缓冲液的首选，确保 pH 值维持在生理范围内 (pH 7 - 9)，适用于广泛的生物体。尽管它很实用，但研究人员在蛋白质研究中需要谨慎，因为 Tris 有可能干扰特定酶的活性。

Tris 碱可能会用作碱度标准品、独立地作为缓冲剂以及作为混合缓冲剂配方（包括 Tris-EDTA (TE) 缓冲液、TAE 缓冲液、TBE 缓冲液等）中的关键成分。其属性包括纯度、基本稳定性和相对不吸湿性，使其成为实验室环境中的可靠选择。在这些环境中，Tris 碱对于制备与生物体液相容的缓冲液是必不可少的，并可作为标准 pH 溶液。它有利于各种实验室程序，例如乳酸脱氢酶测定、原位杂交和从细胞中提取蛋白质。Tris 碱的多功能性扩展到细胞生物学、生物化学和蛋白质研究，对涉及细胞膜通透性和缓冲液制备的研究做出了重大贡献。

应用

Trizma®碱已用于：
  • 用作H缓冲液（细胞解离缓冲液）组分
  • 洗涤和浸润双夹心ELISA免疫酶技术测定反应孔
  • 用作重悬提取并烘干的蛋白质样品的测定缓冲液
  • 制备用于稳定蛋白质的Tris-HCl缓冲液
  • 用作从块茎中提取类胡萝卜素的缓冲液
  • 用作蛋白质印迹前蛋白提取过程的样品缓冲液组分
  • 用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE)的样品缓冲液组分
  • 制备用于磷酸钙(CaP)吸附测定的模拟体液(SBF)
  • 用作在不锈钢(SS)基材上沉积聚多巴胺(PDA)的缓冲液

特点和优势

  • 在 pH 值 7 - 9 范围内有效缓冲，pKa 8.1 (25 °C)
  • 经过测试，确认重金属污染水平较低，确保适合各种应用
  • 可用于细胞生物学和生化研究

其他说明

所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液，温度每降低 1°C，pH 值增加约 0.03 个单位，每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
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法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

可比产品

产品编号
说明
价格

T6687

缓血酸胺, meets USP testing specifications

T6791

Trizma ® 碱, BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

T1378

Sigma 7-9®®, ≥99% (titration), crystalline

T6066

Trizma ® 碱, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

T4661

Trizma ® 碱, ≥99.9% (titration), crystalline

93350

Trizma ® 碱, puriss. p.a., ≥99.7% (T)

93352

Trizma ® 碱, ≥99.0% (T)

93362

Trizma ® 碱, BioUltra, for molecular biology, ≥99.8% (T)

33742

Trizma ® 碱, puriss. p.a., buffer substance, ≥99.5%

154563

三（羟甲基）氨基甲烷, ultrapure grade, ≥99.9%

252859

三（羟甲基）氨基甲烷, ACS reagent, ≥99.8%

T1699

Trizma ® 碱 溶液, 1.5 M

RDD008

Trizma ® 碱, anhydrous, free-flowing, Redi-Dri™, ≥99.9%

建议的防溅射手套

产品编号
说明
价格

通常也和此产品一起购买

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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示例

T1503
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  1. What is the pH of Trizma® base, T1503?

    The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.

  2. What type of electrode should I use to measure the pH of Trizma® base T1503?

    We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.

  3. How can I use T1503 to make a Trizma® buffered solution?

    Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.

  4. Can Product T1503, Trizma® base be used as a standard?

    Trizma base meets the requirements for a primary basimetric standard.

  5. Can I autoclave solutions that contain Product T1503, Trizma® base?

    Yes, Trizma buffered solutions may be autoclaved.  

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

羧肽酶A酶活检测

在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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