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质量水平
描述
aminopeptidase substrate
检测方案
≥99.9% (titration)
形式
crystalline
储存条件
dry at room temperature
技术
ELISA: suitable
protein extraction: suitable
颜色
white
pH值（酸碱度）
10.5-12
有效pH范围
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
溶解性
methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)
吸光度
≤0.05 at 290 nm at 40%
适用性
suitable for Western blot
suitable for electrophoresis
应用
cell analysis
diagnostic assay manufacturing
life science and biopharma
SMILES字符串
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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一般描述
Tris 碱可能会用作碱度标准品、独立地作为缓冲剂以及作为混合缓冲剂配方（包括 Tris-EDTA (TE) 缓冲液、TAE 缓冲液、TBE 缓冲液等）中的关键成分。其属性包括纯度、基本稳定性和相对不吸湿性，使其成为实验室环境中的可靠选择。在这些环境中，Tris 碱对于制备与生物体液相容的缓冲液是必不可少的，并可作为标准 pH 溶液。它有利于各种实验室程序，例如乳酸脱氢酶测定、原位杂交和从细胞中提取蛋白质。Tris 碱的多功能性扩展到细胞生物学、生物化学和蛋白质研究，对涉及细胞膜通透性和缓冲液制备的研究做出了重大贡献。
应用
- 用作H缓冲液（细胞解离缓冲液）组分
- 洗涤和浸润双夹心ELISA免疫酶技术测定反应孔
- 用作重悬提取并烘干的蛋白质样品的测定缓冲液
- 制备用于稳定蛋白质的Tris-HCl缓冲液
- 用作从块茎中提取类胡萝卜素的缓冲液
- 用作蛋白质印迹前蛋白提取过程的样品缓冲液组分
- 用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE)的样品缓冲液组分
- 制备用于磷酸钙(CaP)吸附测定的模拟体液(SBF)
- 用作在不锈钢(SS)基材上沉积聚多巴胺(PDA)的缓冲液
特点和优势
- 在 pH 值 7 - 9 范围内有效缓冲，pKa 8.1 (25 °C)
- 经过测试，确认重金属污染水平较低，确保适合各种应用
- 可用于细胞生物学和生化研究
其他说明
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
法律信息
可比产品
建议的防溅射手套
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
What is the pH of Trizma® base, T1503?
The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.
What type of electrode should I use to measure the pH of Trizma® base T1503?
We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.
How can I use T1503 to make a Trizma® buffered solution?
Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.
Can Product T1503, Trizma® base be used as a standard?
Trizma base meets the requirements for a primary basimetric standard.
Can I autoclave solutions that contain Product T1503, Trizma® base?
Yes, Trizma buffered solutions may be autoclaved.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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实验方案
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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