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Merck
CN

T9285

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

别名:

Tris-EDTA洗涤液, TE 缓冲液 溶液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
41105319
MDL number:
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grade

Molecular Biology

Quality Level

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

General description

Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。

Application

TE缓冲液已用于:
  • 分离、重悬或保存DNA和RNA用于PCR或qPCR
  • 凝胶电泳,包括Northern和Southern免疫印迹分析
  • 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
  • 免疫组化的抗原修复

Preparation Note

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

Other Notes

如需了解生化试剂系列的更多信息,请填写此表


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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

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Sudheesh N et al.
Ticks and tick-borne diseases, 11(3), 101381-101381 (2020-02-06)
Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12



全球贸易项目编号

货号GTIN
T9285-100ML04061838260468