Merck
CN

T9285

Sigma-Aldrich

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

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别名:
TE 缓冲液 溶液
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

等级

for molecular biology

质量水平

无菌性

0.2 μm filtered

形式

liquid

pH值(酸碱度)

7.8-8.2 (1 × in water)

application(s)

sample preparation

异质活性

DNase, RNase, none detected

储存温度

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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相关类别

一般描述

TE缓冲液是分子生物学中最常用的缓冲溶液。“TE”是由Tris(常用pH缓冲液)和EDTA(螯合阳离子的分子)构成。TE(Tris EDTA)缓冲液可溶解DNA和RNA,同时保护其免受降解。TE缓冲液可用于核酸分离(其可能在Northern或Southern印迹杂交之前进行)。

应用

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR
  • gel electrophoresis, including Northern and Southern blotting
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
  • antigen retrieval for immunohistochemistry

组分

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

其他说明

For additional information on our range of Biochemicals, please complete this form.

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

PL860-CGA/SHF-1EA

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1000309185

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批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

    When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

  6. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

    The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

  7. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

    This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

  8. What does 'for molecular biology' mean?

    The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Pak Kin Wong et al.
Analytical chemistry, 76(23), 6908-6914 (2004-12-02)
Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor
Brian O'Farrell et al.
PloS one, 7(10), e48022-e48022 (2012-11-13)
Microbial genotyping increasingly deals with large numbers of samples, and data are commonly evaluated by unstructured approaches, such as spread-sheets. The efficiency, reliability and throughput of genotyping would benefit from the automation of manual manipulations within the context of sophisticated
Emma Lorenzen et al.
Frontiers in immunology, 6, 628-628 (2016-01-07)
International efforts in developing a vaccine against Chlamydia trachomatis have highlighted the need for novel immunization strategies for the induction of genital immunity. In this study, we evaluated an intramuscular (IM) prime/intranasal boost vaccination strategy in a Göttingen Minipig model
Shiraz Tyebji et al.
Cell reports, 31(4), 107573-107573 (2020-04-30)
Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12

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