T9285
Tris-EDTA 缓冲液
100X concentrate for Northern and Southern blotting
别名:
Tris-EDTA洗涤液, TE 缓冲液 溶液
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
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General description
Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物（包括DNA和RNA）的重悬和保存。其中，Tris-HCl是常用缓冲液，EDTA是金属螯合剂。TE缓冲液可溶解核酸，同时保护其免受降解。可用于核酸分离，比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。
Application
TE缓冲液已用于：
- 分离、重悬或保存DNA和RNA用于PCR或qPCR
- 凝胶电泳，包括Northern和Southern免疫印迹分析
- 细菌细胞，用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
- 免疫组化的抗原修复
Preparation Note
TE缓冲液包含1M Tris-HCl（pH约8.0）和0.1M EDTA。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12
Sarah Bøje et al.
Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
Schlenker F et al.
Analytical Chemistry, 93, 10538-10545 (2021)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9285-100ML | 04061838260468 |
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