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Y1501

酵母营养缺陷型培养基补充剂

without uracil

别名:

酵母营养缺陷型合成培养基

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关于此项目

UNSPSC Code:
12352200
NACRES:
ND.02
MDL number:
MFCD01095679
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Quality Level

200

form

powder

application(s)

food and beverages

Application

由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。

Preparation Note

1 L 培养基将补充1.92 g。

Other Notes

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
补充剂包含以下所有成分，除非另有说明：
氨基酸：除亮氨酸外，所有标准氨基酸的浓度均为76 mg/L，亮氨酸含量为 380 mg/L。
其他营养成分：腺嘌呤（18 mg/L）、肌醇（76 mg/L）、对氨基苯甲酸（8 mg/L）

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存储类别

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000508911
0000508911
0000435099
0000435099
0000435093

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实验方案

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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商品

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

酵母转化简介

转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

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全球贸易项目编号

货号GTIN
Y1501-1G04061826260470
Y1501-20G04061838446732