A0349
Anti-l-Afadin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, IHC (f), WB
Citations:
22
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~200 kDa
species reactivity
canine, mouse, rat, human
packaging
antibody small pack of 25 μL
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections., indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells., microarray: suitable, western blot: 1:2,000 using extract of rat brain
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
General description
Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .
Immunogen
Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.
Application
Anti-l-Afadin antibody produced in rabbit has been used in:
- western blotting
- immunofluorescence experiments
- immunocytochemistry
- immunohistochemistry
Biochem/physiol Actions
AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.
Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion
Toret CP, et al.
The Journal of cell biology, 204(2), 265-279 (2014)
Afadin cooperates with Claudin-2 to promote breast cancer metastasis
Tabaries S, et al.
Genes & Development, 33(3-4), 180-193 (2019)
Afadin: a key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
Ikeda W, et al.
The Journal of cell biology, 146(5), 1117-1132 (1999)
Lauren M Popov et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(46), 14337-14342 (2015-10-23)
Staphylococcus aureus is both a transient skin colonizer and a formidable human pathogen, ranking among the leading causes of skin and soft tissue infections as well as severe pneumonia. The secreted bacterial α-toxin is essential for S. aureus virulence in
Genome-wide miRNA analysis identifies miR-188-3p as a novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Pichler M, et al.
Clinical Cancer Research, 23(5), 1323-1333 (2017)
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