A0410
氨基烯丙基-dUTP 钠盐
≥85%, lyophilized powder
别名:
5-[3-氨基烯丙基]-2′-脱氧尿苷 5′-三磷酸盐, AA-dUTP
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关于此项目
经验公式（希尔记法）:
C12H20N3O14P3 · xNa+
化学文摘社编号:
分子量:
523.22 (free acid basis)
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51
Assay:
≥85%
Form:
lyophilized powder
Storage temp.:
−70°C
产品名称
氨基烯丙基-dUTP 钠盐, ≥85%, lyophilized powder
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
SMILES string
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
assay
≥85%
form
lyophilized powder
shipped in
dry ice
storage temp.
−70°C
Quality Level
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Application
氨基烯丙基-dUTP可用于通过聚合酶链式反应（PCR），在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点，用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于：
- 添加至脱氧核苷三磷酸混合物中用于互补DNA（cDNA）的合成
- 生成染色质免疫沉淀（ChIP）探针
- 在RNA的逆转录中用于合成荧光标记的探针
Biochem/physiol Actions
氨基烯丙基-dUTP钠盐/5-（3-氨基烯丙基）-2′-脱氧尿苷5′-三磷酸（aa-dUTP）主要用于合成核酸杂交探针的两步标记法中。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Cox W G and Singer V L
Biotechniques, 36(1), 114-122 (2004)
Miguel A Matilla et al.
Genome biology, 8(9), R179-R179 (2007-09-06)
Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). A global analysis of Pseudomonas putida genes expressed during their interaction
Kevin K Fuller et al.
mBio, 4(2) (2013-03-28)
Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
W Gregory Cox et al.
BioTechniques, 36(1), 114-122 (2004-01-27)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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