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经验公式(希尔记法):
C12H20N3O14P3 · xNa+
化学文摘社编号:
分子量:
523.22 (free acid basis)
MDL编号:
UNSPSC代码:
41106305
NACRES:
NA.51
产品名称
氨基烯丙基-dUTP 钠盐, ≥85%, lyophilized powder
质量水平
方案
≥85%
表单
lyophilized powder
运输
dry ice
储存温度
−70°C
SMILES字符串
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
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应用
氨基烯丙基-dUTP可用于通过聚合酶链式反应(PCR),在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点,用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于:
- 添加至脱氧核苷三磷酸混合物中用于互补DNA(cDNA)的合成
- 生成染色质免疫沉淀(ChIP)探针
- 在RNA的逆转录中用于合成荧光标记的探针
生化/生理作用
氨基烯丙基-dUTP钠盐/5-(3-氨基烯丙基)-2′-脱氧尿苷5′-三磷酸(aa-dUTP)主要用于合成核酸杂交探针的两步标记法中。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical
Miguel A Matilla et al.
Genome biology, 8(9), R179-R179 (2007-09-06)
Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). A global analysis of Pseudomonas putida genes expressed during their interaction
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Cox W G and Singer V L
Biotechniques, 36(1), 114-122 (2004)
Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
Testa A, et al.
The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Eriksson E M, et al.
Plant Physiology, 136(4), 4184-4197 (2004)
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