A0521
α-淀粉酶 来源于人类唾液
Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
别名:
1,4-α-D-葡聚糖-葡萄糖水解酶
类型
Type IX-A
质量水平
表单
lyophilized powder
比活
1,000-3,000 units/mg protein
组成
Protein, 5.0-15.0% biuret
环保替代产品特性
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
UniProt登记号
环保替代产品分类
储存温度
−20°C
基因信息
human ... AMY1A(276)
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一般描述
α-淀粉酶是唾液的主要分泌蛋白成分。它由496个氨基酸组成，具有两种亚型，即糖基化和非糖基化形式。α-淀粉酶基因位于人染色体1p21.1上。α-淀粉酶主要来自于胰腺和唾液。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对淀粉乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅 《Biofiles》的文章。
应用
来自人唾液的α-淀粉酶已可用于通过HPLC（高效液相色谱法）测定α-淀粉酶抑制剂。它还被用于研究人乳低聚糖（HMO）的消化率。
生化/生理作用
α-淀粉酶能够催化寡糖中α-1,4糖苷键的水解。它对于淀粉、糖原和多糖的初始消化至关重要。血清淀粉酶水平升高与急性胰腺炎有关。
外形
冻干粉末，含有(NH4)2SO4 和柠檬酸钠
制备说明
色谱纯化产品
其他说明
在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
HPLC method for measurement of human salivary alpha-amylase inhibition by aqueous plant extracts
Takacs I, et al.
PLoS ONE, 68(2), 127-136 (2017)
M Alberta Araújo et al.
Biomaterials, 25(13), 2687-2693 (2004-01-31)
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation
Structure of human salivary alpha-amylase crystallized in a C-centered monoclinic space group
Fisher SZ, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 62(2), 88-93 (2006)
M J Gnoth et al.
The Journal of nutrition, 130(12), 3014-3020 (2000-12-09)
In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral
Copy number polymorphism of the salivary amylase gene: implications in human nutrition research
Santos JL, et al.
Lifestyle Genomics, 5(3), 117-131 (2012)
实验方案
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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