A0810
内切糖苷酶H 来源于褶皱链霉菌
recombinant, expressed in E. coli, buffered aqueous solution
别名:
β-N-乙酰氨基葡萄糖苷酶 H
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
conjugate
(N-linked)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
糖苷内切酶H或称内切β-N-乙酰氨基葡萄糖苷酶H是一种糖水解酶。它由褶皱链霉菌和其它链霉菌种产生。
Biochem/physiol Actions
糖苷内切酶H参与裂解高甘露糖型糖中聚糖链核心中两个N-乙酰氨基葡萄糖（GlcNAc）残基之间的N-连接的聚糖。
Physical form
溶于含有50 nM NaCl、1 mM EDTA的20mM Tris-HCl，pH 7.5溶液
Other Notes
在37℃、pH5.5条件下，一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。
wgk
WGK 1
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
High-Level Expression of Endo-
Freeze H H and Kranz C
Current Protocols in Molecular Biology, 0 17(3) (2010)
High-Level Expression of Endo-
Wang F, et al.
Testing, 10(3) (2015)
Ulla-Maja Bailey et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 923-924, 16-21 (2013-03-05)
Post-translational modification of proteins with glycosylation is of key importance in many biological systems in eukaryotes, influencing fundamental biological processes and regulating protein function. Changes in glycosylation are therefore of interest in understanding these processes and are also useful as
T Tai et al.
The Journal of biological chemistry, 250(21), 8569-8575 (1975-11-10)
Heterogeneities of the two ovalbumin glycopeptides, (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn, were revealed by borate paper electrophoresis of oligosaccharide alcohols obtained from the glycopeptides by endo-beta-N-acetylglucosaminidase H digestion and NaB3H4 reduction. The structures of the major components of the oligosaccharides were determined
Hironobu Hojo et al.
The Journal of organic chemistry, 77(21), 9437-9446 (2012-07-18)
The complex-type N-linked octasaccharide oxazoline having LacNAc as the nonreducing end sugar was efficiently synthesized using the benzyl-protected LacNAc, mannose, and β-mannosyl GlcNAc units as key building blocks. To achieve a highly β-selective glycosylation with the LacNAc unit, the N-trichloroacetyl
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Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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