Merck
CN

A0810

Sigma-Aldrich

内切糖苷酶H 来源于褶皱链霉菌

recombinant, expressed in E. coli, buffered aqueous solution

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别名:
β-N-乙酰氨基葡萄糖苷酶 H
CAS号:
MDL编号:
NACRES:
NA.32

重组

expressed in E. coli

质量水平

偶联物

(N-linked)

形式

buffered aqueous solution

运输

wet ice

储存温度

2-8°C

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一般描述

糖苷内切酶H或称内切β-N-乙酰氨基葡萄糖苷酶H是一种糖水解酶。它由褶皱链霉菌和其它链霉菌种产生。

生化/生理作用

糖苷内切酶H参与裂解高甘露糖型糖中聚糖链核心中两个N-乙酰氨基葡萄糖(GlcNAc)残基之间的N-连接的聚糖。

单位定义

在37℃、pH5.5条件下,一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。

外形

溶于含有50 nM NaCl、1 mM EDTA的20mM Tris-HCl,pH 7.5溶液

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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High-Level Expression of Endo-
Freeze H H and Kranz C
Current Protocols in Molecular Biology, 0 17(3) (2010)
High-Level Expression of Endo-
Wang F, et al.
Testing, 10(3) (2015)
T Tai et al.
The Journal of biological chemistry, 250(21), 8569-8575 (1975-11-10)
Heterogeneities of the two ovalbumin glycopeptides, (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn, were revealed by borate paper electrophoresis of oligosaccharide alcohols obtained from the glycopeptides by endo-beta-N-acetylglucosaminidase H digestion and NaB3H4 reduction. The structures of the major components of the oligosaccharides were determined
Midori Umekawa et al.
Biochimica et biophysica acta, 1800(11), 1203-1209 (2010-07-22)
An efficient method for synthesizing homogenous glycoproteins is essential for elucidating the structural and functional roles of glycans of glycoproteins. We have focused on the transglycosylation activity of endo-ß-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) as a tool for glycoconjugate syntheses, since
Roger S Zou et al.
Aging, 3(10), 968-984 (2011-10-13)
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form

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