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Merck
CN

A0924

丙烯酰胺/N,N′-亚甲基双丙烯酰胺配制试剂

BioReagent, Molecular Biology, 49:1 (ratio)

别名:

Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
329770639
UNSPSC Code:
41105319
MDL number:
MFCD00080848

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InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioReagent

form

powder

feed ratio

49:1 (ratio)

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

Quality Level

200

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Application

即复溶干粉混合物精确预混合，得到 40% (w/v) 贮备液，用于蛋白质和核酸电泳。该浓度基于丙烯酰胺和双丙烯酰胺的总重量。
Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Features and Benefits

无需称量有毒丙烯酰胺和双丙烯酰胺。

Preparation Note

向容器中缓慢加入规定量的蒸馏水复溶，并溶解内容物。
To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCN7309
SLCK2932
SLCB3104
SLBV3628
SLBH6526V

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访问文档库

PCR Mutation Detection Protocols.
Guilliatt AM
Methods in Molecular Biology (2002)
Bhowmik G
Ana Techniqs in Biotechnology (2011)
Preparation and properties of acrylamide gel for use in electrophoresis.
S RAYMOND et al.
Analytical biochemistry, 1, 391-396 (1960-12-10)

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