biological source
human
Quality Segment
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
626-848 nmol/min·mg
mol wt
A1 subunit ~68 kDa, B1 subunit ~38 kDa, G1 subunit ~40 kDa
solubility
water: soluble
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PRKAA1(5562), PRKAB1(5564), PRKAG1(5571)
General description
研究领域:细胞信号传导
AMP活化蛋白激酶(AMPK)是一种异源三聚体蛋白激酶,由α催化亚基、非催化β亚基和γ亚基组成。
AMP活化蛋白激酶(AMPK)是一种异源三聚体蛋白激酶,由α催化亚基、非催化β亚基和γ亚基组成。
Application
AMP活化蛋白激酶(AMPK)可用于通过生物层干涉(Bio-Layer Interferometry,BLI)结合测定,评估芬氏唐松草碱(thalidezine)与重组活性人 AMPK的结合动力学。
Biochem/physiol Actions
AMP活化蛋白激酶(AMPK)是一种重要的能量感应酶(energy-sensing enzyme),可监测细胞能量状态。响应细胞代谢应激,AMPK被激活并磷酸化和灭活乙酰辅酶A羧化酶(ACC)和3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR),这是调节脂肪酸和胆固醇生物合成的关键酶。
Physical form
50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

