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Merck
CN

A1233

AMPK (α1/β1/γ1),活性,His标记 人

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

别名:

AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
626-848 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
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biological source

human

Quality Segment

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

626-848 nmol/min·mg

mol wt

A1 subunit ~68 kDa, B1 subunit ~38 kDa, G1 subunit ~40 kDa

solubility

water: soluble

UniProt accession no.

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−70°C

Gene Information

General description

研究领域:细胞信号传导

AMP活化蛋白激酶(AMPK)是一种异源三聚体蛋白激酶,由α催化亚基、非催化β亚基和γ亚基组成。

Application

AMP活化蛋白激酶(AMPK)可用于通过生物层干涉(Bio-Layer Interferometry,BLI)结合测定,评估芬氏唐松草碱(thalidezine)与重组活性人 AMPK的结合动力学。

Biochem/physiol Actions

AMP活化蛋白激酶(AMPK)是一种重要的能量感应酶(energy-sensing enzyme),可监测细胞能量状态。响应细胞代谢应激,AMPK被激活并磷酸化和灭活乙酰辅酶A羧化酶(ACC)和3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR),这是调节脂肪酸和胆固醇生物合成的关键酶。

Physical form

50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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