A1233
AMPK (α1/β1/γ1)，活性，His标记 人
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
626-848 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
626-848 nmol/min·mg
mol wt
A1 subunit ~68 kDa, B1 subunit ~38 kDa, G1 subunit ~40 kDa
solubility
water: soluble
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... PRKAA1(5562), PRKAB1(5564), PRKAG1(5571)
General description
研究领域：细胞信号传导
AMP活化蛋白激酶（AMPK）是一种异源三聚体蛋白激酶，由α催化亚基、非催化β亚基和γ亚基组成。
AMP活化蛋白激酶（AMPK）是一种异源三聚体蛋白激酶，由α催化亚基、非催化β亚基和γ亚基组成。
Application
AMP活化蛋白激酶（AMPK）可用于通过生物层干涉（Bio-Layer Interferometry，BLI）结合测定，评估芬氏唐松草碱（thalidezine）与重组活性人 AMPK的结合动力学。
Biochem/physiol Actions
AMP活化蛋白激酶（AMPK）是一种重要的能量感应酶（energy-sensing enzyme），可监测细胞能量状态。响应细胞代谢应激，AMPK被激活并磷酸化和灭活乙酰辅酶A羧化酶（ACC）和3-羟基-3-甲基戊二酰辅酶A还原酶（HMGCR），这是调节脂肪酸和胆固醇生物合成的关键酶。
Physical form
50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
常规特殊物品
此项目有
Thalidezine, a novel AMPK activator, eliminates apoptosis-resistant cancer cells through energy-mediated autophagic cell death
Law BTK
Oncotarget, 8(18), 30077?30091-30077?30091 (2017)
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig S and Shaw RJ
Nature Reviews in Molecular and Cell Biology, 19, 121?135 -121?135 (2018)
AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus.
Yasuhiko Minokoshi et al.
Nature, 428(6982), 569-574 (2004-04-03)
Obesity is an epidemic in Western society, and causes rapidly accelerating rates of type 2 diabetes and cardiovascular disease. The evolutionarily conserved serine/threonine kinase, AMP-activated protein kinase (AMPK), functions as a 'fuel gauge' to monitor cellular energy status. We investigated
D G Hardie et al.
European journal of biochemistry, 246(2), 259-273 (1997-06-01)
A single entity, the AMP-activated protein kinase (AMPK), phosphorylates and regulates in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase (key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively), and probably many additional targets. The kinase is activated by high
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