质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L
运输
ambient
储存温度
2-8°C
一般描述
Ames培养基专为支持视网膜组织的相对短期培养而配制。它适用于体外中枢神经系统组织的维持。
应用
Ames培养基已用于维持大鼠/小鼠/猕猴的眼球，以分离视网膜。
制备说明
补充 1.9 g/L 碳酸氢钠。
配制成每升培养基中含 8.8 克粉末。
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Frans Vinberg et al.
Vision research, 101, 108-117 (2014-06-25)
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
Kasey Rose et al.
Molecular neurodegeneration, 12(1), 28-28 (2017-04-13)
Light exposure triggers movement of certain signaling proteins within the cellular compartments of the highly polarized rod photoreceptor cell. This redistribution of proteins between the inner and outer segment compartments affects the performance and physiology of the rod cell. In
Sabrina Asteriti et al.
Visual neuroscience, 34, E006-E006 (2017-10-03)
Rod-cone gap junctions mediate the so-called "secondary rod pathway", one of three routes that convey rod photoreceptor signals across the retina. Connexin 36 (Cx36) is expressed at these gap junctions, but an unidentified connexin protein also seems to be expressed.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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