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Merck
CN

A1720

G 418 二硫酸盐

powder, non-animal origin, suitable for cell culture, BioReagent

别名:

抗生素G418

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关于此项目

经验公式(希尔记法):
C20H40N4O10 · 2H2SO4
化学文摘社编号:
分子量:
692.71
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.76
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产品名称

G 418 二硫酸盐, powder, BioReagent, suitable for cell culture

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

product line

BioReagent

form

powder

optical activity

[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))

potency

≥720 μg per mg (Dried basis)

mol wt

692.7

packaging

pkg of 1 g
pkg of 25 g
pkg of 5 g

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

antibiotic activity spectrum

Gram-negative bacteria

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

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Application

G418二硫酸盐是一种氨基糖苷类抗生素,结构与庆大霉素相似,可用于筛选和维持利用iNOS启动子构建体以及新霉素抗性基因转染的原核和真核细胞†。不同细胞系具有不同的筛选和维持最佳浓度。 细菌和藻类最多需要5 μg/mL而动物细胞则可能需要300-500 μg/mL。

Biochem/physiol Actions

G-418对于蛋白合成的抑制是在延伸的早期阶段、起始后、翻译起始阶段以及翻译将要起始的阶段之前。

Disclaimer

该产品可2-8°C稳定储存两年。储备溶液可37°C稳定储存8天。

General description

G-418是一种由小单孢菌NRRL 5326菌株产生的氨基糖苷类抗生素。它在体内体外均具有光谱的抗菌活性。它还显示出对原生动物、盲肠阿米巴虫和蠕虫的活性。它通常作为遗传霉素选择性抗生素而被人们所熟知。
化学结构:氨基糖苷类

Other Notes

保持容器密闭并置于干燥通风处。置于干燥处。

Preparation Note

G418粉末可以100 mg/mL溶于水。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

涉药品监管产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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G H Wagman et al.
Antimicrobial agents and chemotherapy, 6(2), 144-149 (1974-08-01)
Antibiotic G-418 is a new aminoglycoside produced as the major component by a new species of Micromonospora, M. rhodorangea NRRL 5326. The antibiotic is prepared by submerged fermentation in a soybean-dextrin medium. Antibiotic G-418 is adsorbed on a cationic-exchange resin
D C Eustice et al.
Antimicrobial agents and chemotherapy, 26(1), 53-60 (1984-07-01)
Tetrahymena thermophila is a eucaryotic organism that is highly susceptible to growth inhibition by aminoglycoside antibiotics. Concentrations of paromomycin, gentamicin G418, and hygromycin B at 22, 10, and 17 microM, respectively, inhibited growth by 50%. A combination of in vitro
Serene R Lai et al.
Methods in molecular biology (Clifton, N.J.), 405, 31-37 (2008-03-29)
RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a
Hillary C Valley et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 18(4), 476-483 (2018-12-20)
Assessment of approved drugs and developmental drug candidates for rare cystic fibrosis (CF)-causing variants of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) requires abundant material from relevant models. Isogenic cell lines harboring CFTR variants in the native genomic context were
Nikolay P Kandul et al.
Nature communications, 11(1), 2106-2106 (2020-05-02)
Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrates proof-of-principle for positive sex selection in D. melanogaster. The system exploits the toxicity of commonly used broad-spectrum antibiotics geneticin and puromycin to kill the non-rescued sex. Sex-specific rescue

商品

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

抗生素杀灭曲线是一项剂量响应实验,通过逐渐增大施加在哺乳动物细胞中的备选抗生素剂量进行测定。

实验方案

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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