A2230
三磷酸腺苷双磷酸酶 来源于马铃薯
High Activity, ATPase ≥600 units/mg protein, lyophilized powder
别名:
腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
应用
三磷酸腺苷双磷酸酶用于水解核苷三磷酸和二磷酸。 Sigma的三磷酸腺苷双磷酸酶已用于血小板聚集的抑制研究。 A2230产品是一种高活性的三磷酸腺苷双磷酸酶(apyrase)。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
反应：ATP → ADP+Pi → AMP+2Pi。
生化/生理作用
三磷酸腺苷双磷酸酶存在于所有真核生物和部分原核生物中。 马铃薯三磷酸腺苷双磷酸酶在调节生长和发育中发挥着至关重要的作用。 Apyrase可参与神经刺激后突触ATP（作为神经递质）的失活，以及抑制ADP诱导的血小板聚集，以防止血栓形成 。 其活性需要二价金属离子，并且使用5mM的钙离子观察到最佳的活性。
包装
按ATP酶单位售卖。
外形
含有琥珀酸钾缓冲盐的冻干粉
制备说明
来源于红土豆
其他说明
在pH 6.5、30℃条件下，一单位酶每分钟可从ATP或ADP释放1.0 μmole无机磷酸。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Xing Liu et al.
Plant physiology, 160(4), 1985-1995 (2012-10-17)
Recent evidence indicates that extracellular nucleotides regulate plant growth. Exogenous ATP has been shown to block auxin transport and gravitropic growth in primary roots of Arabidopsis (Arabidopsis thaliana). Cells limit the concentration of extracellular ATP in part through the activity
Joanne S J Chia et al.
Journal of biomedicine & biotechnology, 2012, 320495-320495 (2012-11-03)
Diabetes mellitus encompasses two distinct disease processes: autoimmune Type 1 (T1D) and nonimmune Type 2 (T2D) diabetes. Despite the disparate aetiologies, the disease phenotype of hyperglycemia and the associated complications are similar. In this paper, we discuss the role of
Moritz Schmelzle et al.
Transplantation, 95(1), 63-69 (2012-12-13)
We have previously demonstrated that CD133 and CD39 are expressed by hematopoietic stem cells (HSC), which are mobilized after liver injury and target sites of injury, limit vascular inflammation, and boost hepatic regeneration. Plasma microparticles (MP) expressing CD39 can block
Martina Nowak-Machen et al.
American journal of respiratory cell and molecular biology, 48(5), 601-609 (2013-01-26)
Critically ill patients are routinely exposed to high concentrations of supplemental oxygen for prolonged periods of time, which can be life-saving in the short term, but such exposure also causes severe lung injury and increases mortality. To address this therapeutic
Thomas J Bushart et al.
American journal of botany, 100(1), 161-174 (2012-10-11)
Gravity regulates the magnitude and direction of a trans-cell calcium current in germinating spores of Ceratopteris richardii. Blocking this current with nifedipine blocks the spore's downward polarity alignment, a polarization that is fixed by gravity ∼10 h after light induces
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