A2230
三磷酸腺苷双磷酸酶 来源于马铃薯
High Activity, ATPase ≥600 units/mg protein, lyophilized powder
别名:
腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
Application
三磷酸腺苷双磷酸酶用于水解核苷三磷酸和二磷酸。 Sigma的三磷酸腺苷双磷酸酶已用于血小板聚集的抑制研究。 A2230产品是一种高活性的三磷酸腺苷双磷酸酶(apyrase)。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
反应：ATP → ADP+Pi → AMP+2Pi。
Biochem/physiol Actions
三磷酸腺苷双磷酸酶存在于所有真核生物和部分原核生物中。 马铃薯三磷酸腺苷双磷酸酶在调节生长和发育中发挥着至关重要的作用。 Apyrase可参与神经刺激后突触ATP（作为神经递质）的失活，以及抑制ADP诱导的血小板聚集，以防止血栓形成 。 其活性需要二价金属离子，并且使用5mM的钙离子观察到最佳的活性。
Packaging
按ATP酶单位售卖。
Physical form
含有琥珀酸钾缓冲盐的冻干粉
Preparation Note
来源于红土豆
Other Notes
在pH 6.5、30℃条件下，一单位酶每分钟可从ATP或ADP释放1.0 μmole无机磷酸。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Yong Zhang et al.
Transplant immunology, 27(4), 175-178 (2012-08-14)
To study the effects and mechanisms of the imbalance in B cell-expressed nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1)-induced ADP degradation on graft injury during acute antibody-mediated rejection (AMR). The acute AMR animal model was established in male NTPDase 1-wild-type Balb/c
Ana Carolina Ribeiro Gomes Maia et al.
Parasitology international, 62(1), 44-52 (2012-09-22)
We identified a shared B domain within nucleoside triphosphate diphosphohydrolases (NTPDases) of plants and parasites. Now, an NTPDase activity not affected by inhibitors of adenylate kinase and ATPases was detected in Leishmania infantum promastigotes. By non-denaturing gel electrophoresis of detergent-homogenized
Gabriane Nascimento Porcino et al.
Experimental parasitology, 132(2), 293-299 (2012-08-28)
Nucleoside triphosphate diphosphohydrolase (NTPDase) activity was recently characterized in Leishmania (Viannia) braziliensis promastigotes (Lb), and an antigenic conserved domain (r82-121) from the specific NTPDase 1 isoform was identified. In this work, mouse polyclonal antibodies produced against two synthetic peptides derived
Maja Milošević et al.
Molecular and cellular biochemistry, 371(1-2), 199-208 (2012-09-08)
Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values
Tsan-Yu Chiu et al.
Plant & cell physiology, 53(11), 1913-1925 (2012-10-05)
Nucleoside triphosphate diphosphohydrolases (NTPDases; apyrases) (EC 3.6.1.5) hydrolyze di- and triphosphate nucleotides, but not monophosphate nucleotides. They are categorized as E-type ATPases, have a broad divalent cation (Mg(2+), Ca(2+)) requirement for activation and are insensitive to inhibitors of F-type, P-type
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