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Merck
CN

A2237

Sigma-Aldrich

磷酸酶,碱性虾

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

别名:

磷酸酶(碱性) 来源于虾, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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化学文摘社编号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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重组

expressed in proprietary host

质量水平

表单

buffered aqueous glycerol solution

浓度

≥900 DEA units/mL

异质活性

RNAse, DNAse, none detected

运输

dry ice

储存温度

−20°C

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一般描述

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

应用

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

生化/生理作用

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

外形

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

分析说明

Protein determined by biuret

其他说明

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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