A2289
白蛋白，四甲基异硫氰酸罗丹明 牛
lyophilized powder
别名:
TRITC-albumin
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关于此项目
Biological source:
bovine
Assay:
≥90% (Lowry)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Impurities:
salt, free
Concentration:
≥90% protein (Lowry)
biological source
bovine
assay
≥90% (Lowry)
form
lyophilized powder
concentration
≥90% protein (Lowry)
extent of labeling
≥0.5 mol TRITC per mol albumin
technique(s)
cell culture | mammalian: suitable
impurities
salt, free
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
General description
Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).
Application
Albumin, Tetramethylrhodamine isothiocyanate has been used:
- in photoinduced release assay of polyethylene beads
- for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
- in fluorescent correlation spectroscopy
Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
Preparation Note
Prepared from crystallized and lyophilized albumin (A4378).
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
13 - Non Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
此项目有
Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
Sokolova V, et al.
Journal of Biological Inorganic Chemistry, 12(2), 174-179 (2007)
Photoresponsive Aqueous Dissolution of Poly (N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Sumaru K, et al.
Biomacromolecules, 19(7), 2913?2922-2913?2922 (2018)
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnology Progress, 22(1), 106-110 (2006)
Correct diffusion coefficients of proteins in fluorescence correlation spectroscopy. Application to tubulin oligomers induced by Mg2+ and Paclitaxel
Krouglova T, et al.
Biophysical Journal, 87(4), 2635-2646 (2004)
Evaluation of fluorescent plasma markers for in vivo microscopy of the microcirculation
Reeves KJ, et al.
Journal of Vascular Research, 49(2), 132-143 (2012)
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