生物来源
bovine
质量水平
方案
≥90% (Lowry)
表单
lyophilized powder
浓度
≥90% protein (Lowry)
标记范围
≥0.5 mol TRITC per mol albumin
技术
cell culture | mammalian: suitable
杂质
salt, free
UniProt登记号
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
一般描述
Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).
应用
Albumin, Tetramethylrhodamine isothiocyanate has been used:
- in photoinduced release assay of polyethylene beads
- for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
- in fluorescent correlation spectroscopy
Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
制备说明
Prepared from crystallized and lyophilized albumin (A4378).
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Photoresponsive Aqueous Dissolution of Poly (N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Sumaru K, et al.
Biomacromolecules, 19(7), 2913?2922-2913?2922 (2018)
Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
Sokolova V, et al.
Journal of Biological Inorganic Chemistry, 12(2), 174-179 (2007)
Correct diffusion coefficients of proteins in fluorescence correlation spectroscopy. Application to tubulin oligomers induced by Mg2+ and Paclitaxel
Krouglova T, et al.
Biophysical Journal, 87(4), 2635-2646 (2004)
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnology Progress, 22(1), 106-110 (2006)
Evaluation of fluorescent plasma markers for in vivo microscopy of the microcirculation
Reeves KJ, et al.
Journal of Vascular Research, 49(2), 132-143 (2012)
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