A2356
碱性磷酸酶 来源于牛肠粘膜
≥5,500 DEA units/mg protein
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum), 碱性磷酸酶
产品名称
碱性磷酸酶 来源于牛肠粘膜, ≥5,500 DEA units/mg protein
form
(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
specific activity
≥5,500 DEA units/mg protein
mol wt
dimer ~160 kDa
concentration
≥10 mg/mL
storage temp.
2-8°C
Quality Level
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Analysis Note
包装规格取决于 DEA 单位
Application
推荐用于抗体和蛋白质缀合的高比活性等级。
碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
Biochem/physiol Actions
该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。
General description
牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工酶，每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
牛肠道碱性磷酸酶是一种二聚化的膜衍生糖蛋白。其存在至少三种异构体，每个单体通常具有两个N-连接的和一个或多个O-连接的聚糖。
Other Notes
在pH 9.8,37℃条件下，一个DEA单位可每分钟水解1 μmole的4-硝基苯基磷酸酯。（一个甘氨酸单位相当于~3个DEA单位）
Physical form
40%甘油溶液，含6 mM Tris、6 mM MgCl2和0.12 mM ZnCl2，pH值约为7.6
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
低风险生物材料
动植物源性产品
此项目有
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实验方案
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
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