A2404
醇氧化酶 溶液 来源于巴斯德毕赤酵母
buffered aqueous solution, 10-40 units/mg protein (biuret)
别名:
酒精：氧氧化还原酶
Application
毕赤酵母来源酒精氧化酶溶液用于棉质果胶甲基酯酶（PME）的酶测定中。它也可以可视化的生物传感器形式固定在聚苯胺（PANI）膜上，以试纸的形式用于测定发酵饮料样品中的乙醇。
乙醇氧化酶可用于研究蛋白向过氧化物酶体的转位。产品 A2404 来自 毕赤酵母 。已用于 Hv-p68 cDNA 克隆的细菌表达和免疫学验证 。
Biochem/physiol Actions
醇氧化酶催化短链初级脂肪醇氧化成各自的醛。对甲醇的亲和力最高。醇氧化酶参与甲醇的代谢，这对利用甲醇作为唯一能源的酵母菌来说很重要。乙醇氧化酶主要存在于过氧化物酶体，但也可能存在于细胞质中。
General description
酒精氧化酶（AOX）是一种均八聚物，分子量为600-kDa。它具有八个黄素腺嘌呤二核苷酸（FAD）辅因子。它属于葡萄糖-甲醇-胆碱（GMC）氧化还原酶家族。
Other Notes
一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。
Physical form
30% 蔗糖溶液，含 0.1 M 磷酸盐缓冲液 (pH 8.0)
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Henry Temple et al.
Nature plants, 8(6), 656-669 (2022-06-11)
Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the
Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties
Liu Q, et al.
PLoS ONE, 8(6), e65131-e65131 (2013)
Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
A I Soldevila et al.
The Journal of biological chemistry, 276(7), 4652-4661 (2000-11-01)
We have cloned and sequenced a novel alcohol oxidase (Hv-p68) from the filamentous fungus Helminthosporium (Cochliobolus) victoriae that copurifies with mycoviral double-stranded RNAs. Sequence analysis revealed that Hv-p68 belongs to the large family of FAD-dependent glucose methanol choline oxidoreductases and
A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples
Kuswandi B, et al.
Sensors, 14(2), 2135-2149 (2014)
实验方案
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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