form
powder
Quality Level
technique(s)
activity assay: suitable
matrix
cross-linked dextran
matrix active group
functional group
bead size
40-125 μm (dry)
pore size
~30,000 Da exclusion limit
operating pH
2-9
capacity
3-4 meq/g ion exchange capacity
compatibility
mode of use mixed weak and strong anion exchange chromatography
General description
Sephadex® 离子交换剂含有具有官能团的交联葡聚糖基质。这些基团通过稳定的醚键连接到基质中的葡萄糖单元。DEAE Sephadex 可用于离子交换色谱法纯化和分离蛋白质。
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
Application
DEAE® Sephadex A-25 已用于：
- 形成 DEAE Sephadex A-25（甲酸盐形式），用于使用辣根植物提取硫代葡萄糖苷（GLS）
- 甘蓝芽中 GLS 含量的测定
- 星形胶质细胞制备和纯化恩多泽平
- 在急性心肌梗死（AMI）患者中鉴定和标记胰岛素激活的一氧化氮合酶抑制剂（蛋白）
Features and Benefits
- 弱阴离子交换剂
- 适用于批处理技术
- 高结合能力
- 对常用的水性缓冲剂添加剂（如 8M 尿素和 6M 盐酸胍）无反应
Legal Information
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
Shu-Kui Wang et al.
Zhonghua nan ke xue = National journal of andrology, 8(3), 173-177 (2002-12-14)
To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2
William F Loomis et al.
The Journal of biological chemistry, 285(28), 21359-21365 (2010-05-11)
Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty acid metabolism and extracellularly as diazepam binding inhibitor, the precursor of endozepine peptides. Two of these peptides, ODN and TTN, bind to the GABA(A) receptor and modulate its sensitivity to
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A25120-100G | 04061833350393 |
| A25120-500G | 04061833350409 |
| A25120-10G | 04061835511679 |
| A25120-50G | 04061833350416 |
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