A2909
兔IgG-琼脂糖
saline suspension
别名:
IgG琼脂糖珠
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Technique(s):
immunoprecipitation (IP): suitable
Conjugate:
agarose conjugate
Application:
IP
Citations:
49
General description
IgG是调节几种免疫应答的糖蛋白抗体。IgG-琼脂糖是一种免疫吸附剂，可用于纯化抗体，去除物种特异性交叉反应抗体，或从抗血清制剂中去除污染抗体。一般来说，可以使用相等树脂体积的IgG-琼脂糖从抗血清制剂中除去交叉反应抗体。然而，树脂与抗血清的比例将随个体应用而变化。免疫球蛋白G（IgG）是免疫球蛋白家族的一部分，并且是一种广泛表达的血清抗体。它的恒定（C）区域包含一条γ 重链。IgG的单体150kDa结构分别由两条相同的重链和两条相同的轻链组成，其分子量分别为50kDa和25kDa。该抗体的一级结构还含有二硫键，可用于连接两条重链、连接重链和轻链、以及链内的连接。IgG可进一步细分为四类，即具有不同重链（γ 1、γ 2、γ 3和γ 4）的IgG1、IgG2、IgG3和IgG4。
Application
兔IgG-琼脂糖已被用于免疫沉淀 和亲和纯化检测。
将与琼脂糖珠交联的兔IgG抗体（20 μl/ml）用于从293T细胞的全细胞提取物中分离RAT标记的蛋白。将交联至琼脂糖珠的兔IgG用于从哺乳动物全细胞提取物中纯化标记蛋白质（0.5至1.0mg总蛋白质结合至10 μl珠子上）。
Physical form
其悬浮于含有防腐剂的0.5M NaCl中。
Other Notes
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
存储类别
10 - Combustible liquids
wgk
WGK 3
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
法规信息
低风险生物材料
此项目有
Emmanuel Vanrobays et al.
RNA (New York, N.Y.), 14(10), 2061-2073 (2008-08-30)
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In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and
A Colley et al.
Molecular and cellular biology, 20(19), 7238-7246 (2000-09-13)
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here we report the analysis of two novel DEAH-box RNA helicases, Dhr1p
Xiaowen Wang et al.
Nature, 524(7566), 481-484 (2015-07-21)
Mitochondria are multifunctional organelles whose dysfunction leads to neuromuscular degeneration and ageing. The multi-functionality poses a great challenge for understanding the mechanisms by which mitochondrial dysfunction causes specific pathologies. Among the leading mitochondrial mediators of cell death are energy depletion
Guixin Du et al.
PloS one, 8(12), e81583-e81583 (2013-12-21)
Human cytomegalovirus protein IE2-p86 exerts its functions through interaction with other viral and cellular proteins. To further delineate its protein interaction network, we generated a recombinant virus expressing SG-tagged IE2-p86 and used tandem affinity purification coupled with mass spectrometry. A
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A2909-2ML | 04061838168214 |
| A2909-5ML | 04061838160591 |
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