Quality Level
biological source
avidin from egg white, enzyme from horseradish
conjugate
peroxidase conjugate
form
lyophilized powder
composition
Protein, ≥70% E1%/280
extent of labeling
0.7-2.0 mol peroxidase per mol avidin
technique(s)
direct ELISA: 1:50,000
solubility
H2O: soluble 1 mg/mL, PBS: soluble
storage temp.
−20°C
General description
亲和素是来自于蛋清的同源四聚体蛋白（68kDa）。这种蛋蛋白质与生物素高度结合。因此，亲和素生物素结合已被用于免疫检测，以检测组织中的抗原定位。亲和素-生物素免疫检测的使用增强了该技术的灵敏度并改善了对低含量抗原的检测。
Application
为了检测小鼠血清中的Ga1缺陷型IgA1抗体，可在96孔板中使用血清进行ELISA检测，其中，血清与生物素化HAA（结合IgA1）一起孵育。然后将HRP偶联的亲和素与血清在37℃孵育40分钟。
亲和素-过氧化物酶已被用于检测抗5-溴脱氧尿苷（抗BrdU）结合和OVA-IgG1。
Physical form
含有柠檬酸盐缓冲液的冻干粉末。
Preparation Note
Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A3151-1MG | 04061835540570 |
| A3151-2MG | 04061833360408 |
| A3151-5MG | 04061833360415 |