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Merck
CN

A3221

碘乙酰胺

Single use vial of 56 mg

别名:

IAA

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线性分子式:
ICH2CONH2
化学文摘社编号:
144-48-9
分子量:
184.96
UNSPSC Code:
12352111
NACRES:
NA.56
PubChem Substance ID:
24890731
EC Number:
205-630-1
Beilstein/REAXYS Number:
1739080
MDL number:
MFCD00008028

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产品名称

碘乙酰胺, Single use vial of 56 mg

suitability

suitable for LC-MS

application(s)

microbiology

InChI

1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)

InChI key

PGLTVOMIXTUURA-UHFFFAOYSA-N

SMILES string

NC(=O)CI

biological source

synthetic (organic)

assay

≥99% (HPLC)

form

powder

packaging

vial of 56 mg (Single use)

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible
only to qualified or authorized persons.)

color

white to off-white

mp

92-95 °C (lit.)

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

Quality Level

200

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相关类别

Application

碘乙酰胺已用于：
  • 防止蛋白质样品发生酶促二硫键还原反应
  • 在细胞生物学研究中抑制甘油醛-3-磷酸脱氢酶，有效阻断ATP生产

Biochem/physiol Actions

碘乙酰胺在肽测序中胱氨酸残基的烷基化试剂。它是活性位点上含有胱氨酸的酶的的一种不可逆抑制剂。它与组氨酸残基的反应速度要慢得多，但这种活性是核糖核酸酶抑制的原因。

Features and Benefits

高品质化合物，适于多个研究领域

General description

2-碘乙酰胺是一种巯基-烷基化试剂，常用于生物化学、细胞培养和蛋白质组学研究中的肽图分析。作用方式与碘乙酸类似，主要通过共价结合蛋白质半胱氨酸残基上的巯基，防止二硫键形成。它在多种应用中发挥重要作用，比如在蛋白质二维电泳中，可作为去泛素化酶(DUB)抑制剂和肽酶抑制剂，减少拖尾并提高分辨率。也常用于肽质谱法从头测序中的样品制备。

它会与蛋白质中的半胱氨酸和组氨酸残基发生特定的相互作用，影响各种酶（如，核糖核酸酶）。与组氨酸缓慢反应的抑制特性令其适合蛋白酶研究（如，半胱氨酸蛋白酶）。碘乙酰胺的优势在于不可逆地抑制酶，形成稳定的蛋白衍生物，是蛋白质结构、蛋白行为和酶抑制研究的有用工具。

Legal Information

与蛋白质组系统合作开发的技术™
Proteome Systems is a trademark of Proteome Systems Ltd

Other Notes

如需了解生化试剂系列的更多信息，请填写此表。

Packaging

密封安瓿包装

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000501415
0000501415
0000456950
0000456950
0000483008

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Tanja Scherrer et al.
Genome biology, 12(1), R3-R3 (2011-01-15)
Glucose inhibition of gluconeogenic growth suppressor 2 protein (Gis2p) and zinc-finger protein 9 (ZNF9) are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown, but human ZNF9 has been reported to bind nucleic acids, and mutations
Kristen L Koterba et al.
Journal of biotechnology, 157(1), 261-267 (2011-12-06)
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We
Mirko Zaffagnini et al.
Antioxidants & redox signaling, 16(1), 17-32 (2011-06-29)
Cysteines (Cys) made acidic by the protein environment are generally sensitive to pro-oxidant molecules. Glutathionylation is a post-translational modification that can occur by spontaneous reaction of reduced glutathione (GSH) with oxidized Cys as sulfenic acids (-SOH). The reverse reaction (deglutathionylation)
Valentina R Minciacchi et al.
Oncotarget, 6(13), 11327-11341 (2015-04-11)
Large oncosomes (LO) are atypically large (1-10 µm diameter) cancer-derived extracellular vesicles (EVs), originating from the shedding of membrane blebs and associated with advanced disease. We report that 25% of the proteins, identified by a quantitative proteomics analysis, are differentially
Elena B Lugli et al.
Arthritis research & therapy, 17, 9-9 (2015-01-21)
Smoking is a well-established risk factor for rheumatoid arthritis (RA), and it has been proposed that smoking-induced citrullination renders autoantigens immunogenic. To investigate this mechanism, we examined human lung tissue from 40 subjects with defined smoking status, with or without
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