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Merck
CN

A3311

牛血清白蛋白 来源于牛血清

powder, BioXtra

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.75
MDL number:
Biological source:
bovine
Assay:
≥98% (agarose gel electrophoresis)
Form:
powder
Technique(s):
cell culture | embryo: suitable
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biological source

bovine

product line

BioXtra

assay

≥98% (agarose gel electrophoresis)

form

powder

Quality Level

packaging

poly bottle of

origin

USA origin

technique(s)

cell culture | embryo: suitable

loss

≤5%

pH

6.5-7.5

solubility

water: soluble (40 mg/ml)

shipped in

ambient

storage temp.

2-8°C

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Application

本白蛋白已经过体外培养应用的验证,如小鼠囊胚和桑椹胚培养。

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白被广泛用作细胞培养基,特别是无血清培养基的添加剂。 它可带来一系列的好处,包括防止氧化损伤和稳定其他培养基成分,如脂肪酸和吡哆醛。

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

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Development (Cambridge, England), 142(13), 2375-2384 (2015-06-21)
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本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

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