A3422
Achromopeptidase 来源于细菌
partially purified powder, ≥20,000 units/mg solid
别名:
ACP
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关于此项目
Specific activity:
≥20,000 units/mg solid
form
partially purified powder
Quality Level
specific activity
≥20,000 units/mg solid
mol wt
27 kDa
foreign activity
Collagenase, present
storage temp.
−20°C
Application
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Biochem/physiol Actions
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Physical form
Partially purified powder.
Other Notes
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
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实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
相关内容
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Ezaki T and Suzuki S
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A3422-50KU | 04061832699523 |
| A3422-25KU | 04061832699509 |