Merck
CN

A3547

Sigma-Aldrich

无色肽酶 来源于细菌

lyophilized powder, ≥1,000 units/mg solid

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别名:
ACP, Lysyl endopeptidase

形式

lyophilized powder

质量水平

比活

≥1,000 units/mg solid

分子量

27 kDa

应用

diagnostic assay manufacturing

运输

dry ice

储存温度

−20°C

应用

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定

生化/生理作用

消化肽酶是赖氨酰内肽酶,其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞,细胞密度 OD600 =0.6,37℃,超过 2 小时。
消化肽酶是赖氨酰内肽酶,分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值:pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞,细胞密度 OD600=0.6,37℃,超过 2 小时 。

单位定义

在 pH8.0,37℃下,使用溶壁微球菌(Micropoccus lysodeikticus)悬浮液作为底物,一个单位的酶每分钟每毫升产生 0.001 的 A600 变化(1cm 光程)。

外形

粗粉

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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J R Buser et al.
Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Adebayo Shittu et al.
Journal of medical microbiology, 53(Pt 1), 51-55 (2003-12-10)
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to
Yuanyuan Hu et al.
World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active

实验方案

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

相关内容

默克提供用于溶解细菌、酵母和昆虫培养物以及植物和哺乳动物细胞培养物和组织样品中蛋白质的蛋白质提取试剂盒、细胞裂解缓冲液和试剂。

Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.

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