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Merck
CN

A3547

无色肽酶 来源于细菌

lyophilized powder, ≥1,000 units/mg solid

别名:

ACP, Lysyl endopeptidase

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关于此项目

NACRES:
NA.54
UNSPSC Code:
12352204

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产品名称

无色肽酶 来源于细菌, lyophilized powder, ≥1,000 units/mg solid

biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

form

lyophilized powder

specific activity

≥1,000 units/mg solid

mol wt

27 kDa

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Other Notes

酶用缓冲液悬浮后，在pH 8.0、37 °C下，1单位每分钟可释放0.4 μg溶壁微球菌（浊度法检测，600nm）。

Physical form

粗粉

Application

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。

Biochem/physiol Actions

消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD600 =0.6，37℃，超过 2 小时。
消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。

signalword

Danger

pictograms

Health hazard
GHS08

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCN3895
SLCN1682
0000506234
0000506234
0000491295

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Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
J R Buser et al.
Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
Yuanyuan Hu et al.
World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active
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实验方案

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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QC Methods

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