A4140
Aequorin from jellyfish (Aequorea sp.)
Type III, solid
biological source
Aequorea sp.
type
Type III
form
solid
usage
mg solid sufficient for ~200 calcium assays
composition
Protein, ~0.5% Bradford
application(s)
detection
storage temp.
−20°C
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General description
Apoaequorin has a mol. wt. approx. 22 kDa, and contains a molecule of coelenterazine hydroperoxide as the chromophoric prosthetic group. Upon binding Ca2+, aequorin decomposes to apoaequorin, coelenteramide, and CO2, and emits light.
Package sizes are based on total solids content
Biochem/physiol Actions
A bioluminescent protein; can be used to measure physiological levels of calcium in serum and subcellular organelles down to 10 μM.
Physical form
Contains buffer salts consisting mainly of sodium EDTA, pH 7.5
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Denis Ottolini et al.
Methods in molecular biology (Clifton, N.J.), 937, 273-291 (2012-09-26)
In the last two decades the study of Ca(2+) homeostasis in living cells has been enhanced by the explosive development of genetically encoded Ca(2+)-indicators. The cloning of the Ca(2+)-sensitive photoprotein aequorin and of the green fluorescent protein (GFP) from the
Nazia Haq et al.
Assay and drug development technologies, 11(2), 93-100 (2012-10-11)
Gap junctions (GJs) are intercellular channels which are composed of the connexin family of proteins that allow electrical and chemical communications and synchronization in tissue ensembles. Evidence suggests that pharmaceutical modulators of these channels may have therapeutic potential or carry
Satoshi Inouye et al.
Protein expression and purification, 83(2), 205-210 (2012-04-28)
Highly purified histidine-tagged aequorin with a reactive cysteine residue (His-Cys4-aequorin) was obtained from the periplasmic space of Escherichia coli cells by nickel-chelate affinity chromatography and hydrophobic chromatography. The procedure yielded 40.3mg of His-Cys4-aequorin from 2L of cultured cells with over
Fen-Fen Lin et al.
The Journal of pharmacology and experimental therapeutics, 345(2), 225-238 (2013-03-12)
Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. There are two key components of this
Daiana Minocci et al.
Biochimica et biophysica acta, 1833(7), 1632-1640 (2013-01-05)
Different optical imaging techniques have been developed to study neuronal activity with the goal of deciphering the neural code underlying neurophysiological functions. Because of several constraints inherent in these techniques as well as difficulties interpreting the results, the majority of
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