A4140
Aequorin from jellyfish (Aequorea sp.)
Type III, solid
生物来源
Aequorea sp.
类型
Type III
表单
solid
用途
mg solid sufficient for ~200 calcium assays
组成
Protein, ~0.5% Bradford
应用
detection
储存温度
−20°C
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一般描述
Apoaequorin has a mol. wt. approx. 22 kDa, and contains a molecule of coelenterazine hydroperoxide as the chromophoric prosthetic group. Upon binding Ca2+, aequorin decomposes to apoaequorin, coelenteramide, and CO2, and emits light.
Package sizes are based on total solids content
生化/生理作用
A bioluminescent protein; can be used to measure physiological levels of calcium in serum and subcellular organelles down to 10 μM.
外形
Contains buffer salts consisting mainly of sodium EDTA, pH 7.5
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Elzbieta Hejchman et al.
The Journal of pharmacology and experimental therapeutics, 343(2), 380-388 (2012-08-16)
We found that amiodarone has potent antifungal activity against a broad range of fungi, potentially defining a new class of antimycotics. Investigations into its molecular mechanisms showed amiodarone mobilized intracellular Ca2+, which is thought to be an important antifungal characteristic
Norbert Mehlmer et al.
Journal of experimental botany, 63(4), 1751-1761 (2012-01-04)
Calcium has long been acknowledged as one of the most important signalling components in plants. Many abiotic and biotic stimuli are transduced into a cellular response by temporal and spatial changes in cellular calcium concentration and the calcium-sensitive protein aequorin
ALSUntangled no. 18: apoaequorin (Prevagen).
Amyotrophic lateral sclerosis & frontotemporal degeneration, 14(1), 78-79 (2012-10-04)
Daiana Minocci et al.
Biochimica et biophysica acta, 1833(7), 1632-1640 (2013-01-05)
Different optical imaging techniques have been developed to study neuronal activity with the goal of deciphering the neural code underlying neurophysiological functions. Because of several constraints inherent in these techniques as well as difficulties interpreting the results, the majority of
Fen-Fen Lin et al.
The Journal of pharmacology and experimental therapeutics, 345(2), 225-238 (2013-03-12)
Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. There are two key components of this
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