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Merck
CN

A4268

α-淀粉酶 来源于猪胰腺

greener alternative

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

别名:

PPA, al1,4 葡聚糖-4-葡萄糖水解酶,, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶

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关于此项目

UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
700-1400 units/mg protein (E1%/280)
Biological source:
Porcine pancreas
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产品名称

α-淀粉酶 来源于猪胰腺, Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

biological source

Porcine pancreas

type

Type I-A

form

saline suspension

specific activity

700-1400 units/mg protein (E1%/280)

mol wt

51-54 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
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sustainability

Greener Alternative Product

technique(s)

activity assay: suitable

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

application(s)

diagnostic assay manufacturing

greener alternative category

storage temp.

2-8°C

Quality Level

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相关类别

Application

α-淀粉酶用于水解α-连接的多糖α键,如淀粉和糖原。Sigma 公司生产的α-淀粉酶已被用于各种植物研究,如拟南芥代谢研究。

Biochem/physiol Actions

α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4)葡聚糖键。天然底物,如淀粉和糖原,被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白,由约 475 个残基的单一多肽链组成,含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca2+

General description

分子质量:51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链(包含2个SH基团和4个二硫键)以及一个紧密结合的Ca2+ (稳定性所必需的)组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0,最适pH为7。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时,该产品经优化可提高能效和防止浪费。有关详细信息,请参阅《Biofiles》的文章

Other Notes

在pH 6.9、温度20 °C条件下,一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

Physical form

悬浮于含 3 mM CaCl2 的 2.9 M NaCl 溶液中。

Preparation Note

2 次结晶

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料
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实验方案

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

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