A4355
Monoclonal Anti-ASPP1 antibody produced in mouse
~2 mg/mL, clone LXO54.2, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Apoptosis-stimulating protein of p53, 1, Anti-PPP1R13B, Anti-protein phosphatase 1 regulatory subunit 13B
产品名称
Monoclonal Anti-ASPP1 antibody produced in mouse, ~2 mg/mL, clone LXO54.2, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LXO54.2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~175 kDa
species reactivity
mouse, human
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of human osteogenic sarcoma, U-2-OS
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PPP1R13B(23368)
mouse ... Ppp1r13b(21981)
Application
Monoclonal Anti-ASPP1 antibody can be used for western blot at 1-2 μg/mL using total cell extract of human osteogenic sarcoma, U-2-OS. The antibody can also be used for microarray and immunoprecipitation assays.
Biochem/physiol Actions
Apoptosis-stimulating protein of p53, 1 (ASPP1) is known to stimulate RAS signalling which subsequently enhances p53-dependent apoptosis in cancer cells. Furthermore, ASPP1 can also induce p53-independent apoptosis by enhancing the apoptotic functions of p63 and p73. Monoclonal Anti-ASPP1 antibody recognizes human and mouse ASPP1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
ASPP1 interacts with p53 to enhance p53-induced apoptosis.
Monoclonal Anti-ASPP1 (mouse IgG1 isotype) is derived from the hybridoma LXO54.2 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant fragment of human ASPP1, amino acids. Apoptosis-stimulating protein of p53, 1 (ASPP1) belongs to the ASPP family of proteins. This protein contains a proline-rich region, four ankyrin repeats, and an SH3 domain in the C-terminal end.
Immunogen
recombinant fragment of human ASPP1 (amino acids 1-308).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Marcus M Schittenhelm et al.
Cell death & disease, 15(1), 25-25 (2024-01-10)
ASPP1 (PPP1R13B) belongs to a family of p53-binding proteins and enhances apoptosis by stimulation of p53-transactivation of selected proapoptotic target genes. It is preferentially expressed in hematopoietic stem cells (HSC) and together with p53 preserves the genomic integrity of the
ASPP proteins specifically stimulate the apoptotic function of p53
Samuels-Lev Y, et al.
Molecular Cell, 8(4), 781-794 (2001)
Daniele Bergamaschi et al.
Molecular and cellular biology, 24(3), 1341-1350 (2004-01-20)
We recently showed that ASPP1 and ASPP2 stimulate the apoptotic function of p53. We show here that ASPP1 and ASPP2 also induce apoptosis independently of p53. By binding to p63 and p73 in vitro and in vivo, ASPP1 and ASPP2
Xingwen Wang et al.
International journal of cancer, 141(7), 1422-1433 (2017-06-29)
Inactivation of p53 has been shown to correlate with drug resistance in tumors. However, in clear cell renal cell carcinoma (ccRCC), p53 is rarely mutated, yet the tumors remain highly insensitive to the conventional chemotherapeutic drugs. The underlying mechanisms responsible
Victor C Y Mak et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 24(4), 522-532 (2010-11-26)
Gestational trophoblastic disease encompasses a spectrum of trophoblastic lesions including true neoplasms such as choriocarcinomas and the potentially malignant hydatidiform moles, which may develop persistent disease requiring chemotherapy. ASPP1, a member of apoptosis-stimulating proteins of p53 (ASPPs), is a proapoptotic
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