conjugate
agarose conjugate
Quality Level
antibody product type
primary antibodies
form
suspension
isotype
IgG12b
capacity
≥0.6 mg/mL, gel binding capacity
storage temp.
−20°C
General description
ANTI-FLAG® M1琼脂糖亲和凝胶由纯化的IgG2B单克隆抗体和琼脂糖共价连接而成。
Application
用于纯化N末端FLAG融合蛋白。由于结合过程不依赖Ca2+，蛋白质能采用含EDTA的缓冲液洗脱，也可以使用FLAG多肽或甘油-HLC缓冲液(pH3)通过标准方法洗脱。ANTI-FLAG M1不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未加工处理的细胞质表达融合蛋白。
该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。
免疫沉淀(IP)：适用
洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA
更多产品信息请访问我们的FLAG®应用页面。
该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。
免疫沉淀(IP)：适用
洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA
更多产品信息请访问我们的FLAG®应用页面。
Biochem/physiol Actions
结合特异性：FLAG 序列
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端
Features and Benefits
- 通常从粗裂解物中纯化融合蛋白，然后只使用一步层析，纯化成单条带
- 用EDTA温和洗脱，将融合蛋白从亲和树脂上洗脱下来。
- FLAG多肽溶液可以用于FLAG融合蛋白温和地，非变性洗脱。
Physical form
球形琼脂糖悬浮在含10 mM磷酸钠、150mM NaCl (pH7.4)、0.02% (w/v) 叠氮化钠的50%甘油溶液中
Other Notes
ANTI-FLAG® M1并不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未处理的细胞质表达融合蛋白。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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相关内容
默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间，以便进一步开展研究。
用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A4596-5ML | 04061833374764 |
| A4596-25ML | 04061833374757 |
| A4596-10ML | 04061833374740 |
| A4596-1ML | 04061838095442 |
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