生物来源
mouse
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
HIS-1, monoclonal
表单
buffered aqueous glycerol solution
技术
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
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一般描述
单克隆抗-多组氨酸-碱性磷酸酶抗体可识别天然和变性还原形式的合成型多组氨酸或多组氨酸标记的融合蛋白。该产物可与由原核pET、pRSET和pTrc表达载体表达的融合蛋白反应。
免疫原
重组聚组氨酸标签融合蛋白。
应用
抗体适用于ELISA、免疫印迹和斑点印迹检测。
生化/生理作用
该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
外形
含有1 mM MgCl2、1% 牛血清白蛋白、50% 甘油和15 mM 叠氮化钠的 0.05MTris 缓冲液,pH 8.0中的溶液
法律信息
该产品受DE 19507166专利及独家授权给QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany的国外同等专利保护。
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
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Optimization of conditions during recombinant protein production for improved yield is a major goal for protein scientists. Typically this is achieved by changing single crucial factor settings one at a time while other factors are kept fixed through trial-and-error experimentation.
Application of a Short, Disordered N-Terminal Flagellin Segment, a Fully Functional Flagellar Type III Export Signal, to Expression of Secreted Proteins
Dobo,J et al.
Applied and Environmental Microbiology, 6(3), 891-899 (2010)
A short disordered N-terminal flagellin segment is a fully functional flagellar type III export signal. Application for secreted protein expression
Dobo,J et al.
Applied and Environmental Microbiology (2009)
Min Yang et al.
Nature chemical biology, 14(12), 1109-1117 (2018-11-14)
The elucidation and prediction of how changes in a protein result in altered activities and selectivities remain a major challenge in chemistry. Two hurdles have prevented accurate family-wide models: obtaining (i) diverse datasets and (ii) suitable parameter frameworks that encapsulate
Richard Glatz et al.
The Journal of biological chemistry, 278(22), 19743-19750 (2003-03-20)
Polydnaviruses are essential for the survival of many Ichneumonoid endoparasitoids, providing active immune suppression of the host in which parasitoid larvae develop. The Cotesia rubecula bracovirus is unique among polydnaviruses in that only four major genes are detected in parasitized
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