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Merck
CN

A5588

抗-多组氨酸−碱性磷酸酶抗体,小鼠单克隆

clone HIS-1, purified from hybridoma cell culture

别名:

聚组氨酸单克隆抗体, 单克隆抗-多组氨酸-碱性磷酸酶 小鼠抗, 6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体

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关于此项目

NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
HIS-1, monoclonal
Application:
WB
Technique(s):
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
Citations:
22
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biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

buffered aqueous glycerol solution

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

Quality Level

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Application

抗体适用于ELISA、免疫印迹和斑点印迹检测。

Biochem/physiol Actions

该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。

General description

单克隆抗-多组氨酸-碱性磷酸酶抗体可识别天然和变性还原形式的合成型多组氨酸或多组氨酸标记的融合蛋白。该产物可与由原核pET、pRSET和pTrc表达载体表达的融合蛋白反应。

Immunogen

重组聚组氨酸标签融合蛋白。

Legal Information

该产品受DE 19507166专利及独家授权给QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany的国外同等专利保护。

Physical form

含有1 mM MgCl2、1% 牛血清白蛋白、50% 甘油和15 mM 叠氮化钠的 0.05MTris 缓冲液,pH 8.0中的溶液

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Application of a Short, Disordered N-Terminal Flagellin Segment, a Fully Functional Flagellar Type III Export Signal, to Expression of Secreted Proteins
Dobo,J et al.
Applied and Environmental Microbiology, 6(3), 891-899 (2010)
Rozbeh Jafari et al.
Microbial cell factories, 10, 34-34 (2011-05-18)
Optimization of conditions during recombinant protein production for improved yield is a major goal for protein scientists. Typically this is achieved by changing single crucial factor settings one at a time while other factors are kept fixed through trial-and-error experimentation.
A short disordered N-terminal flagellin segment is a fully functional flagellar type III export signal. Application for secreted protein expression
Dobo,J et al.
Applied and Environmental Microbiology (2009)
Richard Glatz et al.
The Journal of biological chemistry, 278(22), 19743-19750 (2003-03-20)
Polydnaviruses are essential for the survival of many Ichneumonoid endoparasitoids, providing active immune suppression of the host in which parasitoid larvae develop. The Cotesia rubecula bracovirus is unique among polydnaviruses in that only four major genes are detected in parasitized
Qian-Kun Niu et al.
Molecular plant, 6(4), 1091-1108 (2013-05-29)
Pollen germination and embryogenesis are important to sexual plant reproduction. The processes require a large number of genes to be expressed. Transcription of eukaryotic nuclear genes is accomplished by three conserved RNA polymerases acting in association with a set of

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