A5713
聚组氨酸单克隆抗体
clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins
别名:
6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体
产品名称
聚组氨酸单克隆抗体, clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins
biological source
mouse
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
PBS suspension
isotype
IgG2a
capacity
15-30 nmol/mL binding capacity
suitability
suitable for purification of HIS tagged recombinant proteins
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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Biochem/physiol Actions
该抗体可识别合成聚组氨酸，以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
Immunogen
重组聚组氨酸标签融合蛋白。
Application
Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
General description
This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Eric A Davidson et al.
Current protocols in molecular biology, Chapter 24, Unit 24-Unit 24 (2009-07-04)
This unit describes a protocol for the directed evolution of proteins utilizing in vitro compartmentalization. This method uses a large number of independent in vitro transcription and translation (IVTT) reactions in water droplets suspended in an oil emulsion to enable
Ryan T Cameron et al.
Molecular and cellular neurosciences, 61, 46-55 (2014-05-27)
Up-regulation of Hsp20 protein levels in response to amyloid fibril formation is considered a key protective response against the onset of Alzheimer's disease (AD). Indeed, the physical interaction between Hsp20 and Aβ is known to prevent Aβ oligomerisation and protects
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