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Merck
CN

A6007

Apotryptophanase from Escherichia coli

soluble powder, 75-150 units/mg solid

别名:

Tryptophanase from Escherichia coli, L-Tryptophan indole-lyase (deaminating)

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
75-150 units/mg solid
Biological source:
Escherichia coli
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biological source

Escherichia coli

form

soluble powder

specific activity

75-150 units/mg solid

storage temp.

−20°C

Quality Level

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Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .

Other Notes

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Christian Starkenmann et al.
Journal of agricultural and food chemistry, 59(7), 3358-3365 (2011-03-08)
The objective of this study was to verify whether the volatile organic sulfur compounds recently discovered in bell pepper (Capsicum annuum, L. cultivars), such as the mercapto-ketones: 4-sulfanyl-2-heptanone and 2-sulfanyl-4-heptanone, the mercapto-alcohols: 4-sulfanyl-2-heptanol and 2-sulfanyl-4-heptanol, and heptane-2,4-dithiol, originate from their
Leonardo G Trabuco et al.
Structure (London, England : 1993), 18(5), 627-637 (2010-05-14)
Regulatory nascent chains interact with the ribosomal exit tunnel and modulate their own translation. To characterize nascent chain recognition by the ribosome at the atomic level, extensive molecular dynamics simulations of TnaC, the leader peptide of the tryptophanase operon, inside
Dorota Kuczyńska-Wiśnik et al.
Research in microbiology, 161(10), 847-853 (2010-09-28)
Recent studies have revealed that antibiotics can promote the formation of reactive oxygen species which contribute to cell death. In this study, we report that five different antibiotics known to stimulate production of reactive oxygen species inhibited growth of Escherichia
Zhi-Jun Zhao et al.
Journal of industrial microbiology & biotechnology, 38(12), 1921-1929 (2011-05-05)
Construction and improvement of industrial strains play a central role in the commercial development of microbial fermentation processes. L-tryptophan producers have usually been developed by classical random mutagenesis due to its complicated metabolic network and regulatory mechanism. However, in the
Biochemistry. Nascent proteins caught in the act.
Martin Kampmann et al.
Science (New York, N.Y.), 326(5958), 1352-1353 (2009-12-08)

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