suitability
suitable for chromatography
biological source
plant
form
saline suspension
extent of labeling
≥5 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino of 1,6-diaminohexane
matrix spacer
1 atom
capacity
≥5 mg/mL binding capacity (bovine serum albumin)
storage temp.
2-8°C
Quality Level
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General description
Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.
Application
ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.
Biochem/physiol Actions
ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.
Physical form
Suspension in 0.5 M NaCl containing preservative
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
S Mandjiny et al.
Journal of chromatography, 616(2), 189-195 (1993-07-02)
Histidine, a pseudobiospecific ligand, had been utilized to purify several proteins such as chymosin, acidic protease, carboxypeptidase Y and immunoglobulin G (IgG). A detailed study was undertaken to purify IgG on histidine coupled to aminohexyl Sepharose [A. El-Kak and M.
L G Martini et al.
Pharmaceutical research, 12(11), 1786-1790 (1995-11-01)
The purpose of this study was to investigate the influence of hydration characteristics on the in vitro release of 5-fluorouracil from a swellable matrix prepared using a novel triblock copolymer of poly(epsilon-caprolactone) and poly(oxyethylene). Matrices were prepared by dry compression
Use of stable 6-aminohexyl derivatives for labelling polysaccharides with haptens and for preparing polysaccharide immunoadsorbents.
P L Ey
Journal of immunological methods, 160(1), 135-137 (1993-03-15)
Heterogeneity of antibodies reactive with the dominant antigen of Actinobacillus actinomycetemcomitans.
Nakashima K, et al.
Infection and Immunity, 65, 3794-3798 (1997)
Isa S Duarte et al.
Artificial organs, 30(8), 606-614 (2006-08-17)
This work investigated the potential use of an alternative adsorbent to anti-immunoglobulin E (IgE)-agarose for IgE selective adsorption therapy. A screening of several commercially available adsorbents (Concanavalin A, Lens culinaris[Lc], d-tryptophan, poly-l-lysine, and aminohexyl immobilized on agarose) was done through
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