A6338
乙醛脱氢酶，钾-激活 来源于面包酵母（酿酒酵母）
lyophilized powder, ≥2.0 units/mg protein
别名:
乙醛：NAD[P]+氧化还原酶
表单
lyophilized powder
质量水平
比活
≥2.0 units/mg protein
分子量
228 kDa
组成
Protein, ≥5.0% biuret
运输
dry ice
储存温度
−20°C
一般描述
乙醛脱氢酶是一种四聚体，有几种不同的亚型。该酶在0.01M焦磷酸盐缓冲液中检测，表现为在pH9.3有最大峰值，乙醛为底物。钾离子和半胱氨酸是维持该酶活性必需的条件。铷或NH4+可以用来取代K+, 以及谷胱甘肽可以取代半胱氨酸。锂,Na+,和Cs+抑制反应。乙醛脱氢酶被丙脲，巴豆醛，异氰酸正丙酯，异氰酸环己酯，1-n-丙基-1-[（4-氯苯基）磺酰]-3-n-丙基脲和1-甲基-1-[（4-氯苯基）磺酰]-3-n-丙基脲抑制。该酶可以用来定量血液中的乙醛。
醛脱氢酶（ALDH）存在于细胞核、细胞质、线粒体和内质网中。
应用
醛脱氢酶（ALDH）已用于评估梨提取物对ALDH活性的影响。它还可通过监测烟酰胺腺嘌呤二核苷酸（NAD）的酶促还原，比色测定乙醇。
生化/生理作用
乙醛脱氢酶来自烘焙酵母，通过几种醛催化吡啶核苷酸还原反应。它氧化广泛的底物类型，比如乙醛，甲醛，丙醛，正丁醛，异丁醛，N-戊醛，己醛，苯甲醛，糖醛，D-甘油醛，丙二酸半醛和丁二酸醛。醛脱氢酶用于研究乙醇和异丁醇的生产。通过乙醛脱氢酶预去除醛后，可利用醇脱氢酶监测烟酰胺腺嘌呤二核苷酸（NAD）的酶促还原反应比色测定乙醇浓度。
外形
含有半乳糖，磷酸钾和柠檬酸盐缓冲液和巯基琥珀酸。
制备说明
该酶可以溶解在0.3mg/mL,100mM Tris-HCL缓冲液(pH 8.0), 含有0.02%BSA。
其他说明
一个单位每分钟能够氧化1.0 μmole乙醛生成乙酸，25℃，pH8.0，β-NAD+, 钾和硫醇存在下。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Sergey Stolyar et al.
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Tessa M A Peters et al.
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A two-stage, two-organism process for biohydrogen from glucose
Redwood MD and Macaskie LE
International Journal of Hydrogen Energy, 31(11), 1514-1521 (2006)
Effects and action mechanisms of Korean pear (Pyrus pyrifolia cv. Shingo) on alcohol detoxification.
Ho-Sun Lee et al.
Phytotherapy research : PTR, 26(11), 1753-1758 (2012-03-28)
Korean pear (Pyrus pyrifolia cv. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and
Mark D Redwood et al.
FEMS microbiology letters, 278(1), 48-55 (2007-11-13)
Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two 'uptake' hydrogenase isoenzymes, hydrogenase -1 and -2 (Hyd-1 and -2), and fermentative
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