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Merck
CN

A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

lyophilized powder, ≥20 units/mg protein (biuret)

别名:

N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase

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关于此项目

化学文摘社编号:
NACRES:
NA.32
UNSPSC Code:
12352204
Beilstein/REAXYS Number:
2697172
MDL number:
Specific activity:
≥20 units/mg protein (biuret)
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form

lyophilized powder

Quality Segment

specific activity

≥20 units/mg protein (biuret)

mol wt

~98 kDa

storage temp.

−20°C

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical form

Lyophilized powder containing mannitol and EDTA

Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.


存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号GTIN
A6680-25UN04061832852782