A6941
醇氧化酶 来源于博伊丁假丝酵母
lyophilized powder, 5-15 units/mg protein
别名:
AOD1, AOX, 酒精：氧氧化还原酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-971-3
MDL number:
Specific activity:
5-15 units/mg protein
Biological source:
fungus (Candida boidinii)
产品名称
醇氧化酶 来源于博伊丁假丝酵母, lyophilized powder, 5-15 units/mg protein
biological source
fungus (Candida boidinii)
form
lyophilized powder
specific activity
5-15 units/mg protein
mol wt
octomer 600 kDa by sedimentation equilibrium
solubility
100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)
storage temp.
−20°C
Quality Level
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Application
酒精氧化酶已被用于：
- 催化短链初级脂肪醇氧化成各自的醛。
- 研究酵母的甲醇代谢，如念珠菌、毕赤酵母和汉氏酵母。
- 研究蛋白质到过氧化物酶体的易位。
- 使用酶法测定酒精饮料中的乙醇浓度。
- 开发用于测定酒精的酶电极。
Biochem/physiol Actions
酒精氧化酶对甲醇的亲和力最高。亲和力随着烷基（R）链长度的增加而降低。该酶对仲醇、叔醇、芳香醇或链长超过5个碳原子的脂肪醇几乎没有活性。该产品活性的pH范围是6.5至8.5，最佳pH范围是7.5。酒精氧化酶能促进酒精转化为羰基化合物，同时产生过氧化氢。它也是酵母甲醇利用途径中的第一种酶。
General description
研究领域：细胞信号传导
酒精氧化酶（AOX）是由八个黄素腺嘌呤二核苷酸（FAD）辅助因子组成的同位异构体，属于葡萄糖-甲醇-胆碱（GMC）氧化还原酶家族。AOX1 和 AOX2 基因负责编码 AOX。酒精氧化酶主要定位于过氧物酶体，但也存在于细胞质中。酒精氧化酶是一种 600 kDa 的同源异构体黄蛋白，有八个相等的 74 kDa 亚基；每个亚基都含有一个黄素腺嘌呤二核苷酸（FAD）分子。
酒精氧化酶（AOX）是由八个黄素腺嘌呤二核苷酸（FAD）辅助因子组成的同位异构体，属于葡萄糖-甲醇-胆碱（GMC）氧化还原酶家族。AOX1 和 AOX2 基因负责编码 AOX。酒精氧化酶主要定位于过氧物酶体，但也存在于细胞质中。酒精氧化酶是一种 600 kDa 的同源异构体黄蛋白，有八个相等的 74 kDa 亚基；每个亚基都含有一个黄素腺嘌呤二核苷酸（FAD）分子。
Other Notes
一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。
Physical form
含有磷酸钾缓冲盐、DTE 和稳定剂
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Erol Akyilmaz et al.
Talanta, 61(2), 113-118 (2008-10-31)
An amperometric biosensor based on catalase enzyme for alcohol determination was developed. To construct the biosensor catalase was immobilized by using gelatin and glutaraldehyde on a Clark type dissolved oxygen (DO) probe covered with a teflon membrane which is sensitive
Alcohol oxidase from Candida boidinii.
H Sahm et al.
Methods in enzymology, 89 Pt D, 424-428 (1982-01-01)
H Gülce et al.
Biosensors & bioelectronics, 17(6-7), 517-521 (2002-04-18)
A new enzyme electrode for the determination of alcohols was developed by immobilizing alcohol oxidase in polvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response due to the electrooxidation of enzymatically generated H(2)O(2) was measured at a constant
B Vinet
Clinical chemistry, 33(12), 2204-2208 (1987-12-01)
This method for the specific determination of methanol in serum is based on the following two reactions: (formula; see text) Alcohol oxidase is not specific: it converts all lower alcohols to their corresponding aldehydes; however, formaldehyde dehydrogenase is specific and
H R Waterham et al.
The Journal of cell biology, 139(6), 1419-1431 (1998-02-12)
Alcohol oxidase (AOX), the first enzyme in the yeast methanol utilization pathway is a homooctameric peroxisomal matrix protein. In peroxisome biogenesis-defective (pex) mutants of the yeast Pichia pastoris, AOX fails to assemble into active octamers and instead forms inactive cytoplasmic
实验方案
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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