A7510
腺苷 5′-三磷酸酶 来源于猪大脑皮质
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
别名:
ATP 磷酸水解酶, ATP酶
生物来源
Porcine cerebral cortex
质量水平
描述
Sodium and Potassium-Activated
表单
lyophilized powder
比活
≥0.3 units/mg protein, pH 7.8
组成
Protein, 5.0-25.0% biuret
技术
inhibition assay: suitable
储存温度
−20°C
基因信息
pig ... ATP1A1(397481), ATP1B1(396898)
应用
ATP酶用于从ATP中释放无机磷。来自猪大脑皮质的ATP酶已用于Na / K-ATP酶活性的抑制测定。
生化/生理作用
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。蟾酥毒基和其他强心类固醇可能抑制 Na +/K +-atp 酶活性，诱导 HL60、U937 和 ML1 细胞分化。
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。 它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。
外形
含有90%蔗糖、0.4%EDTA Na 4+和0.06%NaCl的冻干粉末
制备说明
碘化钠提取部分，Nakao 等人， Biochem. Biophys. Res. Commun., 19, 755 (1965).
其他说明
如果存在Na+, K+和Mg2+，在 pH值为 7.8、37 C 条件下，每分钟从 ATP 释放 1.0 μmol 无机磷
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Aung Ko Win et al.
Journal of the National Cancer Institute, 105(4), 274-279 (2013-02-07)
Lynch syndrome is an autosomal dominantly inherited disorder caused by germline mutations in DNA mismatch repair (MMR) genes. Previous studies have shown that MMR gene mutation carriers are at increased risk of colorectal, endometrial, and several other cancers following an
An siRNA screen for NFAT activation identifies septins as coordinators of store-operated Ca2+ entry.
Sonia Sharma et al.
Nature, 499(7457), 238-242 (2013-06-25)
The STIM1-ORAI1 pathway of store-operated Ca(2+) entry is an essential component of cellular Ca(2+) signalling. STIM1 senses depletion of intracellular Ca(2+) stores in response to physiological stimuli, and relocalizes within the endoplasmic reticulum to plasma-membrane-apposed junctions, where it recruits and
Amita Kaundal et al.
Bio-protocol, 10(15), e3705-e3705 (2021-03-05)
ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA+-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by spectrophotometric absorption at 360 nm
Marco Chiabudini et al.
Molecular and cellular biology, 34(21), 4062-4076 (2014-08-27)
Ribosome stalling is an important incident enabling the cellular quality control machinery to detect aberrant mRNA. Saccharomyces cerevisiae Hbs1-Dom34 and Ski7 are homologs of the canonical release factor eRF3-eRF1, which recognize stalled ribosomes, promote ribosome release, and induce the decay
Daniel D Buchanan et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 32(2), 90-100 (2013-12-11)
Clinicopathologic data from a population-based endometrial cancer cohort, unselected for age or family history, were analyzed to determine the optimal scheme for identification of patients with germline mismatch repair (MMR) gene mutations. Endometrial cancers from 702 patients recruited into the
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