产品名称
JumpStart™ Taq抗体, Adds hot-start capabilities to any Taq DNA Polymerase
biological source
mouse
conjugate
unconjugated
clone
monoclonal
form
liquid
feature
hotstart
concentration
1.1 mg/mL
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
JumpStart™Taq 抗体适用于：
- 细胞色素阻断核酸（LNA）探针检测
- 连接介导的聚合酶链式反应
- 病毒滴度的实时定量 PCR 分析
- 复杂基因组或 cDNA 模板的 PCR 扩增
- 放大拷贝数非常低的目标
- 用于在同一反应管中进行多个热循环（>35）和多个引物对的 PCR 扩增
General description
JumpStart™ Taq 抗体为热启动 PCR 提供了一种有效的方法。该抗体是一种对 Taq DNA 聚合酶具有特异性的中和单克隆抗体。当抗体与 Taq 结合时，该酶就失去活性。在热循环的第一个变性步骤，酶-抗体复合物解离，JumpStart Taq 抗体变得不起作用。一旦抗体变性，Taq DNA 聚合酶的活性就会恢复，并且酶在 PCR 反应过程中正常发挥作用。JumpStart Taq 通常用于防止不良扩增产物。
Other Notes
JumpStart Taq抗体，1.1μg/1μL
稀释缓冲液在1 mL小管中提供
稀释缓冲液在1 mL小管中提供
Packaging
提供稀释缓冲液。
Preparation Note
通过JumpStart Taq抗体的1次测试使两个单位的Taq DNA聚合酶失活。
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
低风险生物材料
常规特殊物品
此项目有
D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
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