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Merck
CN

A8264

4-氨基邻苯二甲酰肼

≥98% (HPLC), powder

别名:

6-氨基-2,3-二氢-1,4-酞嗪二酮, 异鲁米诺

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关于此项目

经验公式(希尔记法):
C8H7N3O2
化学文摘社编号:
分子量:
177.16
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
164804
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产品名称

4-氨基邻苯二甲酰肼,

InChI

1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13)

SMILES string

Nc1ccc2C(=O)NNC(=O)c2c1

InChI key

HUDPLKWXRLNSPC-UHFFFAOYSA-N

assay

≥98% (HPLC)

form

powder

mp

300 °C (lit.)

solubility

2 M NH4OH: 50 mg/mL, clear to slightly hazy

Quality Level

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Application

4-氨基邻苯二甲酰肼(异鲁米诺)已用于:
  • 假结核耶氏菌感染后骨髓(BM)中性粒细胞的化学发光法活性氧(ROS)检测
  • 克雷伯氏肺炎菌(K. pneumonia)暴露后中性粒细胞的活性氧检测
  • 甲酰-蛋氨酸-亮氨酸-脯氨酸刺激后中性粒细胞的活性氧检测

Biochem/physiol Actions

4-氨基邻苯二甲酰肼或异鲁米诺是由酞嗪环构成的化学发光试剂,亲水性更高于鲁米诺。异鲁米诺在商业上广泛用于各种体外免疫分析,是可用于研究活性氧的放大性化学发光试剂。异鲁米诺也可用作电发光生物传感器(ECL)和微生物快速检测探针。

Features and Benefits

能发出化学荧光。

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A new isoluminol reagent for chemiluminescence labeling of proteins
Palmioli A, et al.
Tetrahedron Letters, 54, 4446-4450 (2013)
Anna-Lena Stenfeldt et al.
Luminescence : the journal of biological and chemical luminescence, 22(5), 507-510 (2007-07-05)
A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol-amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide.
Zian Lin et al.
Analytical chemistry, 78(15), 5322-5328 (2006-08-02)
A new method for pressurized capillary electrochromatography (pCEC) coupling with chemiluminescence (CL) detection using a modified on-column coaxial flow detection interface was developed. To evaluate the feasibility and reliability of the experimental setup, the typical CL compounds luminol and isoluminol
Chiedzo M Mpofu et al.
Gastroenterology, 133(5), 1487-1498 (2007-10-09)
Crohn's disease (CD) is mimicked by inherited phagocyte disorders and is associated with circulating antibodies against yeast mannan (anti-Saccharomyces cerevisiae antibody; ASCA). We speculated that mannans might impair phagocyte function. S cerevisiae mannan was assessed for its effects on human
Chien-Wen S Kuo et al.
Journal of immunology (Baltimore, Md. : 1950), 187(1), 361-371 (2011-06-08)
Infection of human cells by human T cell leukemia virus type 1 (HTLV-1) is mediated by the viral envelope glycoproteins. The gp46 surface glycoprotein binds to cell surface receptors, including heparan sulfate proteoglycans, neuropilin 1, and glucose transporter 1, allowing

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