A8353
Anti-hABH3 antibody, Mouse monoclonal
clone hABH3-99, purified from hybridoma cell culture
别名:
Anti-Human AlkB Homologue 3, Monoclonal Anti-hABH3 antibody produced in mouse
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关于此项目
Conjugate:
unconjugated
Clone:
hABH3-99, monoclonal
Application:
ARR, ELISA (i), IHC, IP, WB
Citations:
3
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hABH3-99, monoclonal
form
buffered aqueous solution
mol wt
antigen ~33 kDa
species reactivity
rat, hamster, monkey, bovine, canine, human, mouse
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of 293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Monkey ... ALKBH3(715921)
human ... ALKBH3(221120)
mouse ... Alkbh3(69113)
rat ... Alkbh3(362169)
General description
Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm. Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH).
Application
- Mouse Monoclonal Anti-hABH3 has been used in:
- western blot
- immunohistochemistry
- immunoprecipitation
- indirect enzyme linked immunosorbent assay (ELISA)
Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.
Biochem/physiol Actions
Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.
hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Aas PA, et al.
Nature, 421(6925), 859-859 (2003)
Valentina Cetica et al.
Journal of neuro-oncology, 94(2), 195-201 (2009-03-18)
Alkylating agents, commonly used for brain tumor therapy, induce DNA and RNA lesions that, if not repaired, drive cells to apoptosis. Thus, cellular mechanisms that are responsible for nucleic acid repair are possibly involved in drug resistance. This work analyzes
Norihisa Uemura et al.
Cancer science, 100(9), 1612-1622 (2009-06-30)
To identify the molecular background of esophageal cancer, we conducted a proteomics study using an antibody microarray consisting of 725 antibodies and surgical specimens from three cases. The microarray analysis identified 24 proteins with aberrant expression in esophageal cancer compared
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